Discoveries of Exoribonuclease-Resistant Structures of Insect-Specific Flaviviruses Isolated in Zambia

Wastika, Christida E.; Harima, Hayato; Sasaki, Michihito; Hang'ombe, Bernard M.; Eshita, Yuki; Qiu, Yongjin; Hall, William W.; Wolfinger, Michael T.; Sawa, Hirofumi; Orba, Yasuko

Discoveries of Exoribonuclease-Resistant Structures of Insect-Specific Flaviviruses Isolated in Zambia

Wastika, Christida E.; Harima, Hayato; Sasaki, Michihito et al.

Viruses-Basel, 2020, 12(9), 1017

Permalink : https://hdl.handle.net/2115/79920

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アクセス権	open access
URI	https://hdl.handle.net/2115/79920
タイトル	Discoveries of Exoribonuclease-Resistant Structures of Insect-Specific Flaviviruses Isolated in Zambia
著者	Wastika, Christida E. ; Harima, Hayato ; Sasaki, Michihito ; Hang'ombe, Bernard M. ; Eshita, Yuki ; Qiu, Yongjin ; Hall, William W. ; Wolfinger, Michael T. ; Sawa, Hirofumi ; Orba, Yasuko
著者名 Wastika, Christida E.
著者名 Harima, Hayato
著者名 Sasaki, Michihito
著者名 Hang'ombe, Bernard M.
著者名 Eshita, Yuki
著者名 Qiu, Yongjin
著者名 Hall, William W.
著者名 Wolfinger, Michael T.
著者名 Sawa, Hirofumi
e-Rad_Researcher 60507169 著者名 Orba, Yasuko
言語	英語
キーワード	Barkedji virus
	Barkedji-like virus
	dISFVs
	5 '-UTR
	3 '-UTR
	RNA secondary structure
発行日	2020-09
出版者	MDPI
収録物名	Viruses-Basel
巻(号)	12(9)
ページ	1017
抄録	To monitor the arthropod-borne virus transmission in mosquitoes, we have attempted both to detect and isolate viruses from 3304 wild-caught female mosquitoes in the Livingstone (Southern Province) and Mongu (Western Province) regions in Zambia in 2017. A pan-flavivirus RT-PCR assay was performed to identify flavivirus genomes in total RNA extracted from mosquito lysates, followed by virus isolation and full genome sequence analysis using next-generation sequencing and rapid amplification of cDNA ends. We isolated a newly identified Barkedji virus (BJV Zambia) (10,899 nt) and a novel flavivirus, tentatively termed Barkedji-like virus (BJLV) (10,885 nt) fromCulexspp. mosquitoes which shared 96% and 75% nucleotide identity with BJV which has been isolated in Israel, respectively. These viruses could replicate in C6/36 cells but not in mammalian and avian cell lines. In parallel, a comparative genomics screening was conducted to study evolutionary traits of the 5 '- and 3 '-untranslated regions (UTRs) of isolated viruses. Bioinformatic analyses of the secondary structures in the UTRs of both viruses revealed that the 5 '-UTRs exhibit canonical stem-loop structures, while the 3 '-UTRs contain structural homologs to exoribonuclease-resistant RNAs (xrRNAs), SL-III, dumbbell, and terminal stem-loop (3 ' SL) structures. The function of predicted xrRNA structures to stop RNA degradation by Xrn1 exoribonuclease was further proved by the in vitro Xrn1 resistance assay.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution 4.0 International
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.3390/v12091017