Effects of pentosan polysulfate on cell proliferation, cell cycle progression and cyclin-dependent kinases expression in canine articular chondrocytes

Akaraphutiporn, Ekkapol; Bwalya, Eugene C.; Kim, Sangho; Sunaga, Takafumi; Echigo, Ryosuke; Okumura, Masahiro

Effects of pentosan polysulfate on cell proliferation, cell cycle progression and cyclin-dependent kinases expression in canine articular chondrocytes

Akaraphutiporn, Ekkapol; Bwalya, Eugene C.; Kim, Sangho et al.

Journal of veterinary medical science, 2020, 82(8), 1209-1218

Permalink : https://hdl.handle.net/2115/79922

このアイテムのアクセス数:757件（2026-08-19 06:38 集計）

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https://doi.org/10.1292/jvms.20-0091			16

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アクセス権	open access
URI	https://hdl.handle.net/2115/79922
タイトル	Effects of pentosan polysulfate on cell proliferation, cell cycle progression and cyclin-dependent kinases expression in canine articular chondrocytes
著者	Akaraphutiporn, Ekkapol ; Bwalya, Eugene C. ; Kim, Sangho ; Sunaga, Takafumi ; Echigo, Ryosuke ; Okumura, Masahiro
著者名 Akaraphutiporn, Ekkapol
著者名 Bwalya, Eugene C.
著者名 Kim, Sangho
著者名 Sunaga, Takafumi
著者名 Echigo, Ryosuke
e-Rad_Researcher 80260397 著者名 Okumura, Masahiro
言語	英語
キーワード	articular chondrocyte
	cell cycle
	cyclin-dependent kinases
	osteoarthritis
	pentosan polysulfate
発行日	2020-08
出版者	公益社団法人日本獣医学会 (The Japanese Society of Veterinary Science)
収録物名	Journal of veterinary medical science
巻(号)	82(8)
ページ	1209-1218
抄録	Pentosan polysulfate (PPS) is a semi-synthetic sulfated polysaccharide compound which has been shown the benefits on therapeutic treatment for osteoarthritis (OA) and has been proposed as a disease modifying osteoarthritis drugs (DMOADs). This study investigated the effects of PPS on cell proliferation, particularly in cell cycle modulation and phenotype promotion of canine articular chondrocytes (AC). Canine AC were treated with PPS (0-80 mu g/ml) for 24, 48 and 72 hr. The effect of PPS on cell viability, cell proliferation and cell cycle distribution were analyzed by MTT assay, DNA quantification and flow cytometry. Chondrocyte phenotype was analyzed by quantitative real-time PCR (qPCR) and glycosaminoglycan (GAG) quantification. PPS significantly reduced AC proliferation through cell cycle modulation particularly by maintaining a significantly higher proportion of chondrocytes in the G1 phase and a significantly lower proportion in the S phase of the cell cycle in a concentration- and time-dependent manner. While the proportion of chondrocytes in G1 phase corresponded with the significant downregulation of cyclin-dependent kinase (CDK) 1 and 4. Furthermore, the study confirms that PPS promotes a chondrogenic phenotype of AC through significant upregulation of collagen type II (Col2A1) mRNA and GAG synthesis. The effect of PPS on the inhibition of chondrocyte proliferation while promoting a chondrocyte phenotype could be beneficial in the early stages of OA treatment, which transient increase in proliferative activity of chondrocytes with subsequent phenotypic shift and less productive in an essential component of extracellular matrix (ECM) is observed.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
PISSN	0916-7250
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1292/jvms.20-0091