Receptor-Mediated Host Cell Preference of a Bat-Derived Filovirus, Lloviu Virus

Takadate, Yoshihiro; Manzoor, Rashid; Saito, Takeshi; Kida, Yurie; Maruyama, Junki; Kondoh, Tatsunari; Miyamoto, Hiroko; Ogawa, Hirohito; Kajihara, Masahiro; Igarashi, Manabu; Takada, Ayato

Receptor-Mediated Host Cell Preference of a Bat-Derived Filovirus, Lloviu Virus

Takadate, Yoshihiro; Manzoor, Rashid; Saito, Takeshi et al.

Microorganisms, 2020, 8(10), 1530

Permalink : https://hdl.handle.net/2115/79958

このアイテムのアクセス数:569件（2026-10-17 16:20 集計）

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https://doi.org/10.3390/microorganisms8101530			28

論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/79958
タイトル	Receptor-Mediated Host Cell Preference of a Bat-Derived Filovirus, Lloviu Virus
著者	Takadate, Yoshihiro ; Manzoor, Rashid ; Saito, Takeshi ; Kida, Yurie ; Maruyama, Junki ; Kondoh, Tatsunari ; Miyamoto, Hiroko ; Ogawa, Hirohito ; Kajihara, Masahiro ; Igarashi, Manabu ; Takada, Ayato
著者名 Takadate, Yoshihiro
著者名 Manzoor, Rashid
e-Rad_Researcher 40153811 著者名 Saito, Takeshi
著者名 Kida, Yurie
著者名 Maruyama, Junki
著者名 Kondoh, Tatsunari
著者名 Miyamoto, Hiroko
e-Rad_Researcher 80455237 著者名 Ogawa, Hirohito
e-Rad_Researcher 70711894 著者名 Kajihara, Masahiro
e-Rad_Researcher 10374240 著者名 Igarashi, Manabu
e-Rad_Researcher 10292062 著者名 Takada, Ayato
言語	英語
キーワード	filovirus
	lloviu virus
	Miniopterus sp
	bat
	insectivorous bat
	host range
	Niemann–Pick C1
	glycoprotein
発行日	2020-10
出版者	MDPI
収録物名	Microorganisms
巻(号)	8(10)
ページ	1530
抄録	Lloviu virus (LLOV), a bat-derived filovirus that is phylogenetically distinct from human pathogenic filoviruses such as Ebola virus (EBOV) and Marburg virus (MARV), was discovered in Europe. However, since infectious LLOV has never been isolated, the biological properties of this virus remain poorly understood. We found that vesicular stomatitis virus (VSV) pseudotyped with the glycoprotein (GP) of LLOV (VSV-LLOV) showed higher infectivity in one bat (Miniopterus sp.)-derived cell line than in the other bat-derived cell lines tested, which was distinct from the tropism of VSV pseudotyped with EBOV (VSV-EBOV) and MARV GPs. We then focused on the interaction between GP and Niemann-Pick C1 (NPC1) protein, one of the cellular receptors of filoviruses. We introduced the Miniopterus bat and human NPC1 genes into NPC1-knockout Vero E6 cells and their susceptibilities to the viruses were compared. The cell line expressing the bat NPC1 showed higher susceptibility to VSV-LLOV than that expressing human NPC1, whereas the opposite preference was seen for VSV-EBOV. Using a site-directed mutagenesis approach, amino acid residues involved in the differential tropism were identified in the NPC1 and GP molecules. Our results suggest that the interaction between GP and NPC1 is an important factor in the tropism of LLOV to a particular bat species.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution 4.0 International
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.3390/microorganisms8101530