The effect of zebularine on the heat-activated retrotransposon ONSEN in Arabidopsis thaliana and Vigna angularis

Boonjing, Patwira; Masuta, Yukari; Nozawa, Kosuke; Kato, Atsushi; Ito, Hidetaka

The effect of zebularine on the heat-activated retrotransposon ONSEN in Arabidopsis thaliana and Vigna angularis

Boonjing, Patwira; Masuta, Yukari; Nozawa, Kosuke et al.

Genes & Genetic Systems, 2020, 95(4), 165-172

Permalink : https://hdl.handle.net/2115/79977

このアイテムのアクセス数:496件（2026-08-18 13:27 集計）

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ファイル	フォーマット	サイズ	閲覧回数	説明
https://doi.org/10.1266/ggs.19-00046			15

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アクセス権	open access
URI	https://hdl.handle.net/2115/79977
タイトル	The effect of zebularine on the heat-activated retrotransposon ONSEN in Arabidopsis thaliana and Vigna angularis
著者	Boonjing, Patwira ; Masuta, Yukari ; Nozawa, Kosuke ; Kato, Atsushi ; Ito, Hidetaka
著者名 Boonjing, Patwira
著者名 Masuta, Yukari
著者名 Nozawa, Kosuke
著者名 Kato, Atsushi
e-Rad_Researcher 70582295 著者名 Ito, Hidetaka
言語	英語
キーワード	adzuki bean
	Arabidopsis
	heat stress
	ONSEN
	zebularine
発行日	2020-08
出版者	日本遺伝学会(The Genetics Society of Japan)
収録物名	Genes & Genetic Systems
巻(号)	95(4)
ページ	165-172
抄録	The Ty1/copia-like retrotransposon ONSEN is conserved among Brassica species, as well as in beans, including adzuki bean (Vigna angularis (Willd.) Ohwi & Ohashi), which is one of the economically important crops in Japan. ONSEN has acquired a heat-responsive element that is recognized by plant heat stress defense factors, resulting in its transcription and the production of full-length extrachromosomal DNA under conditions with elevated temperatures. DNA methylation plays an important role in regulating the activation of this transposon in plants. Therefore, chemical inhibition of DNA methyltransferases has been utilized to study the effect of DNA methylation on transposon activation. To understand the effect of DNA methylation on ONSEN activation, Arabidopsis thaliana and adzuki bean seedlings were treated with zebularine, which is known to be an effective chemical demethylation agent. The results showed that ONSEN transcription levels were upregulated in zebularine-treated plants. Extrachromosomal DNA of ONSEN also accumulated in the treated plants.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
PISSN	1341-7568
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1266/ggs.19-00046