Chemical Labeling Assisted Detection and Identification of Short Chain Fatty Acid Esters of Hydroxy Fatty Acid in Rat Colon and Cecum Contents

Gowda, Siddabasave Gowda B.; Gowda, Divyavani; Liang, Chongsheng; Li, Yonghan; Kawakami, Kentaro; Fukiya, Satoru; Yokota, Atsushi; Chiba, Hitoshi; Hui, Shu-Ping

Chemical Labeling Assisted Detection and Identification of Short Chain Fatty Acid Esters of Hydroxy Fatty Acid in Rat Colon and Cecum Contents

Gowda, Siddabasave Gowda B.; Gowda, Divyavani; Liang, Chongsheng et al.

Metabolites, 2020, 10(10), 398

Permalink : https://hdl.handle.net/2115/79991

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アクセス権	open access
URI	https://hdl.handle.net/2115/79991
タイトル	Chemical Labeling Assisted Detection and Identification of Short Chain Fatty Acid Esters of Hydroxy Fatty Acid in Rat Colon and Cecum Contents
著者	Gowda, Siddabasave Gowda B. ; Gowda, Divyavani ; Liang, Chongsheng ; Li, Yonghan ; Kawakami, Kentaro ; Fukiya, Satoru ; Yokota, Atsushi ; Chiba, Hitoshi ; Hui, Shu-Ping
著者名 Gowda, Siddabasave Gowda B.
著者名 Gowda, Divyavani
著者名 Liang, Chongsheng
著者名 Li, Yonghan
著者名 Kawakami, Kentaro
e-Rad_Researcher 10370157 著者名 Fukiya, Satoru
e-Rad_Researcher 50220554 著者名 Yokota, Atsushi
e-Rad_Researcher 70197622 著者名 Chiba, Hitoshi
e-Rad_Researcher 90337030 著者名 Hui, Shu-Ping
言語	英語
キーワード	FAHFAs
	Chemical labeling
	DMED
	High-fat diet
	murine model
	LC-MS
発行日	2020-10
出版者	MDPI
収録物名	Metabolites
巻(号)	10(10)
ページ	398
抄録	Branched fatty acid esters of hydroxy fatty acids (FAHFAs) are novel endogenous lipids with important physiological functions in mammals. We previously identified a new type of FAHFAs, named short-chain fatty acid esterified hydroxy fatty acids (SFAHFAs), with acetyl or propyl esters of hydroxy fatty acids of carbon chains, C >= 20. However, sensitive determination of SFAHFAs is still a challenge, due to their high structural similarity and low abundance in biological samples. This study employs one-step chemical derivatization following total lipid extraction using 2-dimethylaminoethylamine (DMED) for enhanced detection of SFAHFAs. The labeled extracts were subjected to ultrahigh performance liquid chromatography coupled to linear ion trap quadrupole-Orbitrap mass spectrometry (UHPLC/LTQ-Orbitrap MS). Our results demonstrated that the detection sensitivities of SFAHFAs increased after DMED labeling, and is highly helpful in discovering six additional novel SFAHFAs in the cecum and colon contents of WKAH/HKmSlc rats fed with normal and high-fat diet (HFD). The identified DMED labeled SFAHFAs were characterized by their detailed MS/MS analysis, and their plausible fragmentation patterns were proposed. The concentrations of SFAHFAs were significantly reduced in the cecum of HFD group compared to the control. Hence, the proposed method could be a promising tool to apply for the enhanced detection of SFAHFAs in various biological matrices, which in turn facilitate the understanding of their sources, and physiological functions of these novel lipids.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution 4.0 International
PISSN	2218-1989
関連情報	PMID 33050007
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.3390/metabo10100398