Improved Isolation of Uncultured Anaerobic Bacteria using Medium Prepared with Separate Sterilization of Agar and Phosphate

Kato, Souichiro; Terashima, Mia; Yama, Ayano; Sato, Megumi; Kitagawa, Wataru; Kawasaki, Kosei; Kamagata, Yoichi

Improved Isolation of Uncultured Anaerobic Bacteria using Medium Prepared with Separate Sterilization of Agar and Phosphate

Kato, Souichiro; Terashima, Mia; Yama, Ayano et al.

Microbes and environments, 2021, 35(1), ME19060

Permalink : https://hdl.handle.net/2115/80146

このアイテムのアクセス数:527件（2026-08-08 04:50 集計）

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https://doi.org/10.1264/jsme2.ME19060			11

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アクセス権	open access
URI	https://hdl.handle.net/2115/80146
タイトル	Improved Isolation of Uncultured Anaerobic Bacteria using Medium Prepared with Separate Sterilization of Agar and Phosphate
著者	Kato, Souichiro ; Terashima, Mia ; Yama, Ayano ; Sato, Megumi ; Kitagawa, Wataru ; Kawasaki, Kosei ; Kamagata, Yoichi
e-Rad_Researcher 30597787 著者名 Kato, Souichiro
著者名 Terashima, Mia
著者名 Yama, Ayano
著者名 Sato, Megumi
著者名 Kitagawa, Wataru
著者名 Kawasaki, Kosei
著者名 Kamagata, Yoichi
言語	英語
キーワード	culturability
	anaerobic bacteria
	wastewater treatment plant
	agar medium preparation
	phosphate
発行日	2021-01-13
出版者	Japanese Society of Microbial Ecology, Department of Bioresource Science
収録物名	Microbes and environments
巻(号)	35(1)
ページ	ME19060
抄録	We previously demonstrated that a simple modification in the preparation of agar media, i.e., autoclaving phosphate and agar separately (termed the "PS protocol"), improved the culturability of aerobic microorganisms by reducing the generation of reactive oxygen species. We herein investigated the effects of the PS protocol on the cultivation of anaerobic microorganisms using sludge from a wastewater treatment system as a microbial source. The application of the PS protocol increased colony numbers and the frequency of phylogenctically novel isolates under aerobic, nitrate reduction, and fermentation conditions. The PS protocol is useful for isolating both aerobic and anaerobic microorganisms.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
PISSN	1342-6311
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1264/jsme2.ME19060