open access

https://hdl.handle.net/2115/80258

Biosynthesis of Random-Homo Block Copolymer Poly[Glycolate-ran-3-Hydroxybutyrate (3HB)]-b-Poly(3HB) Using Sequence-Regulating Chimeric Polyhydroxyalkanoate Synthase in Escherichia coli

英語

chimeric enzyme

2020-12-08

Frontiers Media

Frontiers in bioengineering and biotechnology

8

612991

Glycolate (GL)-containing polyhydroxyalkanoate (PHA) was synthesized in Escherichia coli expressing the engineered chimeric PHA synthase PhaC(AR) and coenzyme A transferase. The cells produced poly[GL-co-3-hydroxybutyrate (3HB)] with the supplementation of GL and 3HB, thus demonstrating that PhaC(AR) is the first known class I PHA synthase that is capable of incorporating GL units. The triad sequence analysis using H-1 nuclear magnetic resonance indicated that the obtained polymer was composed of two distinct regions, a P(GL-ran-3HB) random segment and P(3HB) homopolymer segment. The random segment was estimated to contain a 71 mol% GL molar ratio, which was much greater than the value (15 mol%) previously achieved by using PhaC1(P)(s)STQK. Differential scanning calorimetry analysis of the polymer films supported the presence of random copolymer and homopolymer phases. The solvent fractionation of the polymer indicated the presence of a covalent linkage between these segments. Therefore, it was concluded that PhaC(AR) synthesized a novel random-homo block copolymer, P(GL-ran-3HB)-b-P(3HB).

学術雑誌論文

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Creative Commons Attribution 4.0 International

2296-4185