Bone enhancing effect of titanium-binding proteins isolated from bovine bone and implanted into rat calvaria with titanium scaffold

Kuboki, Yoshinori; Furusawa, Toshitake; Sato, Masaaki; Sun, Yongkun; Unuma, Hidero; Abe, Shigeaki; Fujisawa, Ryuichi; Akasaka, Tsukasa; Watari, Fumio; Takita, Hiroko; Sammons, Rachel

Bone enhancing effect of titanium-binding proteins isolated from bovine bone and implanted into rat calvaria with titanium scaffold

Kuboki, Yoshinori; Furusawa, Toshitake; Sato, Masaaki et al.

Bio-Medical Materials and Engineering, 2014, 24(3), 1539-1548

Permalink : https://hdl.handle.net/2115/80377

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https://doi.org/10.3233/BME-140958			28

論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/80377
タイトル	Bone enhancing effect of titanium-binding proteins isolated from bovine bone and implanted into rat calvaria with titanium scaffold
著者	Kuboki, Yoshinori ; Furusawa, Toshitake ; Sato, Masaaki ; Sun, Yongkun ; Unuma, Hidero ; Abe, Shigeaki ; Fujisawa, Ryuichi ; Akasaka, Tsukasa ; Watari, Fumio ; Takita, Hiroko ; Sammons, Rachel
e-Rad_Researcher 00014001 著者名 Kuboki, Yoshinori
著者名 Furusawa, Toshitake
著者名 Sato, Masaaki
著者名 Sun, Yongkun
著者名 Unuma, Hidero
e-Rad_Researcher 40374566 著者名 Abe, Shigeaki
著者名 Fujisawa, Ryuichi
e-Rad_Researcher 00360917 著者名 Akasaka, Tsukasa
e-Rad_Researcher 70158682 著者名 Watari, Fumio
e-Rad_Researcher 30125330 著者名 Takita, Hiroko
著者名 Sammons, Rachel
言語	英語
キーワード	Bone phosphoprotein
	titanium
	bone formation
	chromatography
発行日	2014
出版者	IOS Press
収録物名	Bio-Medical Materials and Engineering
巻(号)	24(3)
ページ	1539-1548
抄録	Based on our previous finding that a chromatography with titanium beads selectively binds phosphoproteins, including caseins, phosvitin and dentin phosphoproteins, we investigated whether bone phosphoproteins also bind to titanium. Bovine bone matrix proteins were extracted with 2 M urea/PBS after demineralization. The 2 M urea extract was directly applied to the titanium chromatography column as reported. The chromatogram showed an initial large peak at breakthrough position (non-binding fraction) and a smaller second peak eluted later (titanium-binding fraction). Both peaks were analyzed by SDS polyacrylamide gel electrophoresis. Stains-all staining which preferentially identifies phospho-proteins revealed that the first peak contained no positively stained band, while the second peak showed 4 or 5 distinctive bands indicative of bone phosphoproteins. To investigate the biological functions of the titanium-binding bone proteins (TiBP), we implanted them into calvaria of rats, combined with titanium web (TW), a highly porous titanium scaffold of thin titanium-fibers. Bone TiBP induced significantly enhanced bone formation, and new bone appeared connected directly to titanium fibers, accompanied by active blood vessel formations. Control TW alone did not induce bone formation within the titanium framework. These results demonstrate that the bone titanium-binding proteins include phosphoproteins which enhance bone formation when implanted into bone with titanium.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	The final publication is available at IOS Press through http://dx.doi.org/10.3233/BME-140958
PISSN	0959-2989
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.3233/BME-140958