Involvement of N- and C-terminal region of recombinant cervid prion protein in its reactivity to CWD and atypical BSE prions in real-time quaking-induced conversion reaction in the presence of high concentrations of tissue homogenates

Suzuki, Akio; Sawada, Kazuhei; Yamasaki, Takeshi; Denkers, Nathaniel D.; Mathiason, Candace K.; Hoover, Edward A.; Horiuchi, Motohiro

Involvement of N- and C-terminal region of recombinant cervid prion protein in its reactivity to CWD and atypical BSE prions in real-time quaking-induced conversion reaction in the presence of high concentrations of tissue homogenates

Suzuki, Akio; Sawada, Kazuhei; Yamasaki, Takeshi et al.

Prion, 2020, 14(1), 283-295

Permalink : https://hdl.handle.net/2115/80399

このアイテムのアクセス数:476件（2026-08-12 15:43 集計）

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https://doi.org/10.1080/19336896.2020.1858694			36

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アクセス権	open access
URI	https://hdl.handle.net/2115/80399
タイトル	Involvement of N- and C-terminal region of recombinant cervid prion protein in its reactivity to CWD and atypical BSE prions in real-time quaking-induced conversion reaction in the presence of high concentrations of tissue homogenates
著者	Suzuki, Akio ; Sawada, Kazuhei ; Yamasaki, Takeshi ; Denkers, Nathaniel D. ; Mathiason, Candace K. ; Hoover, Edward A. ; Horiuchi, Motohiro
著者名 Suzuki, Akio
著者名 Sawada, Kazuhei
著者名 Yamasaki, Takeshi
著者名 Denkers, Nathaniel D.
著者名 Mathiason, Candace K.
著者名 Hoover, Edward A.
e-Rad_Researcher 30219216 著者名 Horiuchi, Motohiro
言語	英語
キーワード	bovine spongiform encephalopathy
	cervid prion protein
	chronic wasting disease
	prion
	real-time quaking-induced conversion
発行日	2020-01-01
出版者	Taylor & Francis
収録物名	Prion
巻(号)	14(1)
ページ	283-295
抄録	The real-time quaking-induced conversion (RT-QuIC) reaction is a sensitive and specific method for detecting prions. However, inhibitory factors present in tissue homogenates can easily interfere with this reaction. To identify the RT-QuIC condition under which low levels of chronic wasting disease (CWD) and bovine spongiform encephalopathy (BSE) prions can be detected in the presence of high concentrations of brain tissue homogenates, reactivities of various recombinant prion proteins (rPrPs) were tested. Among the tested rPrPs, recombinant cervid PrP (rCerPrP) showed a unique reactivity: the reactivity of rCerPrP to CWD and atypical BSE prions was not highly affected by high concentrations of normal brain homogenates. The unique reactivity of rCerPrP disappeared when the N-terminal region (aa 25-93) was truncated. Replacement of aa 23-149 of mouse (Mo) PrP with the corresponding region of CerPrP partially restored the unique reactivity of rCerPrP in RT-QuIC. Replacement of the extreme C-terminal region of MoPrP aa 219-231 to the corresponding region of CerPrP partially conferred the unique reactivity of rCerPrP to rMoPrP, suggesting the involvement of both N- and C-terminal regions. Additionally, rCer(N)-Mo-Cer(C)PrP, a chimeric PrP comprising CerPrP aa 25-153, MoPrP aa 150-218, and CerPrP aa 223-233, showed an additive effect of the N- and C-terminal regions. These results provide a mechanistic implication for detecting CWD and atypical BSE prions using rCerPrP and are useful for further improvements of RT-QuIC.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution 4.0 International
PISSN	1933-6896
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1080/19336896.2020.1858694