Alterations of Glycosphingolipid Glycans and Chondrogenic Markers during Differentiation of Human Induced Pluripotent Stem Cells into Chondrocytes

Xu, Liang; Hanamatsu, Hisatoshi; Homan, Kentaro; Onodera, Tomohiro; Miyazaki, Takuji; Furukawa, Jun-ichi; Hontani, Kazutoshi; Tian, Yuan; Baba, Rikiya; Iwasaki, Norimasa

Alterations of Glycosphingolipid Glycans and Chondrogenic Markers during Differentiation of Human Induced Pluripotent Stem Cells into Chondrocytes

Xu, Liang; Hanamatsu, Hisatoshi; Homan, Kentaro et al.

Biomolecules, 2020, 10(12), 1622

Permalink : https://hdl.handle.net/2115/80465

このアイテムのアクセス数:675件（2026-08-27 06:43 集計）

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論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/80465
タイトル	Alterations of Glycosphingolipid Glycans and Chondrogenic Markers during Differentiation of Human Induced Pluripotent Stem Cells into Chondrocytes
著者	Xu, Liang ; Hanamatsu, Hisatoshi ; Homan, Kentaro ; Onodera, Tomohiro ; Miyazaki, Takuji ; Furukawa, Jun-ichi ; Hontani, Kazutoshi ; Tian, Yuan ; Baba, Rikiya ; Iwasaki, Norimasa
著者名 Xu, Liang
e-Rad_Researcher 70734185 著者名 Hanamatsu, Hisatoshi
e-Rad_Researcher 40823331 著者名 Homan, Kentaro
e-Rad_Researcher 70547174 著者名 Onodera, Tomohiro
著者名 Miyazaki, Takuji
e-Rad_Researcher 30374193 著者名 Furukawa, Jun-ichi
e-Rad_Researcher 10805279 著者名 Hontani, Kazutoshi
著者名 Tian, Yuan
e-Rad_Researcher 40815742 著者名 Baba, Rikiya
e-Rad_Researcher 30322803 著者名 Iwasaki, Norimasa
言語	英語
キーワード	cartilage injury
	glycomics
	glycosphingolipid
	human induced pluripotent stem cells
	chondrocytes
	aminolysis-SALSA
発行日	2020-12
出版者	MDPI
収録物名	Biomolecules
巻(号)	10(12)
ページ	1622
抄録	Due to the limited intrinsic healing potential of cartilage, injury to this tissue may lead to osteoarthritis. Human induced pluripotent stem cells (iPSCs), which can be differentiated into chondrocytes, are a promising source of cells for cartilage regenerative therapy. Currently, however, the methods for evaluating chondrogenic differentiation of iPSCs are very limited; the main techniques are based on the detection of chondrogenic genes and histological analysis of the extracellular matrix. The cell surface is coated with glycocalyx, a layer of glycoconjugates including glycosphingolipids (GSLs) and glycoproteins. The glycans in glycoconjugates play important roles in biological events, and their expression and structure vary widely depending on cell types and conditions. In this study, we performed a quantitative GSL-glycan analysis of human iPSCs, iPSC-derived mesenchymal stem cell like cells (iPS-MSC like cells), iPS-MSC-derived chondrocytes (iPS-MSC-CDs), bone marrow-derived mesenchymal stem cells (BMSCs), and BMSC-derived chondrocytes (BMSC-CDs) using glycoblotting technology. We found that GSL-glycan profiles differed among cell types, and that the GSL-glycome underwent a characteristic alteration during the process of chondrogenic differentiation. Furthermore, we analyzed the GSL-glycome of normal human cartilage and found that it was quite similar to that of iPS-MSC-CDs. This is the first study to evaluate GSL-glycan structures on human iPS-derived cartilaginous particles under micromass culture conditions and those of normal human cartilage. Our results indicate that GSL-glycome analysis is useful for evaluating target cell differentiation and can thus support safe regenerative medicine.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution 4.0 International
PISSN	2218-273X
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.3390/biom10121622