Nicotine directly affects milk production in lactating mammary epithelial cells concurrently with inactivation of STAT5 and glucocorticoid receptor in vitro

Kobayashi, Ken; Tsugami, Yusaku; Suzuki, Norihiro; Suzuki, Takahiro; Nishimura, Takanori

Nicotine directly affects milk production in lactating mammary epithelial cells concurrently with inactivation of STAT5 and glucocorticoid receptor in vitro

Kobayashi, Ken; Tsugami, Yusaku; Suzuki, Norihiro et al.

Toxicology in vitro, 2020, 63, 104741

Permalink : https://hdl.handle.net/2115/80562

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アクセス権	open access
URI	https://hdl.handle.net/2115/80562
タイトル	Nicotine directly affects milk production in lactating mammary epithelial cells concurrently with inactivation of STAT5 and glucocorticoid receptor in vitro
著者	Kobayashi, Ken ; Tsugami, Yusaku ; Suzuki, Norihiro ; Suzuki, Takahiro ; Nishimura, Takanori
e-Rad_Researcher 30449003 著者名 Kobayashi, Ken
著者名 Tsugami, Yusaku
著者名 Suzuki, Norihiro
著者名 Suzuki, Takahiro
e-Rad_Researcher 10237729 著者名 Nishimura, Takanori
言語	英語
キーワード	Mammary epithelial cell
	Nicotine
	Milk production
	Tight junction
	Apoptosis
発行日	2020-03
出版者	Elsevier
収録物名	Toxicology in vitro
巻(号)	63
ページ	104741
抄録	Nicotine from tobacco smoke is absorbed into the bloodstream and transferred into breast milk in breastfeeding mothers. Smoking causes a decrease in breast milk volume, adverse changes to the milk composition, and a shortened lactation period. Breast milk is produced by mammary epithelial cells (MECs) in mammary glands during lactation. However, it remains unclear whether nicotine directly affects milk production in lactating MECs. To address this issue, we prepared a culture model with high milk production ability and less-permeable tight junctions (TJs) by seeding mouse MECs on a cell culture insert. Lactating MECs showed expression of α2, α3, β2, and β4 of nicotinic acetylcholine receptors. The high concentration of nicotine at 10-100 μM inhibited β-casein secretion and caused abnormal localization of TJ proteins. We subsequently investigated whether nicotine at a physiological concentration could affect lactating MECs. Nicotine at 1.0 μM directly inhibited α- and β-casein secretion in lactating MECs concurrently with inactivation of STAT5 and glucocorticoid receptor without affecting the TJ barrier. Nicotine treatment also induced MEC apoptosis concurrently with inactivation of Akt. These results support the adverse effects of nicotine on breastfeeding in smoking mothers.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	© 2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	0887-2333
EISSN	1879-3177
NCID	AA10678594
関連情報	PMID 31783125
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.tiv.2019.104741