Antibacterial and Antibiofilm Photodynamic Activities of Lysozyme-Au Nanoclusters/Rose Bengal Conjugates

Okamoto, Ichie; Miyaji, Hirofumi; Miyata, Saori; Shitomi, Kanako; Sugaya, Tsutomu; Ushijima, Natsumi; Akasaka, Tsukasa; Enya, Satoshi; Saita, Satoshi; Kawasaki, Hideya

Antibacterial and Antibiofilm Photodynamic Activities of Lysozyme-Au Nanoclusters/Rose Bengal Conjugates

Okamoto, Ichie; Miyaji, Hirofumi; Miyata, Saori et al.

ACS Omega, 2021, 6(13), 9279-9290

Permalink : https://hdl.handle.net/2115/80980

このアイテムのアクセス数:645件（2026-08-19 03:29 集計）

閲覧可能ファイル

ファイル	フォーマット	サイズ	閲覧回数	説明
2021 Okamoto ACS Omega	pdf	5.15 MB	487

論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

アクセス権	open access
URI	https://hdl.handle.net/2115/80980
タイトル	Antibacterial and Antibiofilm Photodynamic Activities of Lysozyme-Au Nanoclusters/Rose Bengal Conjugates
著者	Okamoto, Ichie ; Miyaji, Hirofumi ; Miyata, Saori ; Shitomi, Kanako ; Sugaya, Tsutomu ; Ushijima, Natsumi ; Akasaka, Tsukasa ; Enya, Satoshi ; Saita, Satoshi ; Kawasaki, Hideya
著者名 Okamoto, Ichie
e-Rad_Researcher 50372256 著者名 Miyaji, Hirofumi
著者名 Miyata, Saori
e-Rad_Researcher 70881449 著者名 Shitomi, Kanako
e-Rad_Researcher 10211301 著者名 Sugaya, Tsutomu
著者名 Ushijima, Natsumi
e-Rad_Researcher 00360917 著者名 Akasaka, Tsukasa
著者名 Enya, Satoshi
著者名 Saita, Satoshi
e-Rad_Researcher 50322285 著者名 Kawasaki, Hideya
言語	英語
発行日	2021-03-23
出版者	American Chemical Society
収録物名	ACS Omega
巻(号)	6(13)
ページ	9279-9290
抄録	Antibacterial photodynamic therapy (aPDT) utilizes reactive oxygen species such as singlet oxygen (1O2) and free radicals via photosensitizers, which are light and light-sensitive agents, to reduce bacterial infections. It has been utilized as a treatment for dental diseases in place of antibiotic therapies. However, aPDT does not always cause the desired therapeutic effect due to the instability of organic photosensitizers and the formation of bacterial biofilms. To promote the antibacterial and antibiofilm effects of aPDT, we have proposed a lysozyme (Lys)-gold nanoclusters (Au NCs)/rose bengal (Lys-Au NCs/RB) conjugate as a novel photosensitizer. This conjugate was found to effectively impede the growth of both gram-positive and gram-negative bacteria when exposed to white light-emitting diode (LED) irradiation. The photoexcited Lys-Au NCs/RB showed significantly higher antibacterial activity than photoexcited Lys-Au NCs or RB alone. The synergistic effect is a result of the combination of Lys (an antibacterial protein) and enhanced 1O2 generation related to resonance energy transfer (RET) in the Au NCs/RB conjugate. Photoexcited Lys-Au NCs/RB increased the effects of aPDT in a dose- and time-dependent manner. Furthermore, the photoexcited Lys-Au NCs/RB successfully decreased Streptococcus mutans biofilm formation. However, in contrast, it did not have a negative effect on the proliferation, adhesion, or spread of mammalian cells, indicating low cytotoxicity. Lys-Au NCs/RB is a novel photosensitizer with low cytotoxicity that is capable of bacterial inactivation and the suppression of biofilm formation, and could help to improve dental treatments in the future.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution 4.0 International
PISSN	2470-1343
関連情報	PMID 33842797
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1021/acsomega.1c00838