Mastomys natalensis is a possible natural rodent reservoir for encephalomyocarditis virus

Kishimoto, Mai; Hang'ombe, Bernard M.; Hall, William W.; Orba, Yasuko; Sawa, Hirofumi; Sasaki, Michihito

Mastomys natalensis is a possible natural rodent reservoir for encephalomyocarditis virus

Kishimoto, Mai; Hang'ombe, Bernard M.; Hall, William W. et al.

Journal of General Virology, 2021, 102(3), 001564

Permalink : https://hdl.handle.net/2115/81006

このアイテムのアクセス数:1,082件（2026-08-10 18:07 集計）

閲覧可能ファイル

ファイル	フォーマット	サイズ	閲覧回数	説明
Mastomys_natalensis_is_a_possible_natural_rodent_reservoir_for_encephalomyocarditis_virus	pdf	1.88 MB	614
upplimental_table	xlsx	20.8 KB	179

論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

アクセス権	open access
URI	https://hdl.handle.net/2115/81006
タイトル	Mastomys natalensis is a possible natural rodent reservoir for encephalomyocarditis virus
著者	Kishimoto, Mai ; Hang'ombe, Bernard M. ; Hall, William W. ; Orba, Yasuko ; Sawa, Hirofumi ; Sasaki, Michihito
著者名 Kishimoto, Mai
著者名 Hang'ombe, Bernard M.
著者名 Hall, William W.
e-Rad_Researcher 60507169 著者名 Orba, Yasuko
e-Rad_Researcher 30292006 著者名 Sawa, Hirofumi
e-Rad_Researcher 70609403 著者名 Sasaki, Michihito
言語	英語
キーワード	Encephalomyocarditis virus
	Rodent
	Mastomys natalensis
	Reservoir
	Zambia
発行日	2021-02-03
出版者	Society for General Microbiology
収録物名	Journal of General Virology
巻(号)	102(3)
ページ	001564
抄録	Encephalomyocarditis virus (EMCV) infects a wide range of hosts and can cause encephalitis, myocarditis, reproductive disorders and diabetes mellitus in selected mammalian species. As for humans, EMCV infection seems to occur by the contact with animals and can cause febrile illnesses in some infected patients. Here we isolated EMCV strain ZM12/14 from a natal multimammate mouse (Mastomys natalensis: M. natalensis) in Zambia. Pairwise sequence similarity of the ZM12/14 P1 region consisting of antigenic capsid proteins showed the highest similarity of nucleotide (80.7 %) and amino acid (96.2%) sequence with EMCV serotype 1 (EMCV-1). Phylogenetic analysis revealed that ZM12/14 clustered into EMCV-1 at the P1 and P3 regions but segregated from known EMCV strains at the P2 region, suggesting a unique evolutionary history. Reverse transcription PCR (RT-PCR) screening and neutralizing antibody assays for EMCV were performed using collected tissues and serum from various rodents (n=179) captured in different areas in Zambia. We detected the EMCV genome in 19 M. natalensis (19/179=10.6 %) and neutralizing antibody for EMCV in 33 M. natalensis (33/179=18.4 %). However, we did not detect either the genome or neutralizing antibody in other rodent species. High neutralizing antibody litres (≧320) were observed in both RT-PCR-negative and -positive animals. Inoculation of ZM12/14 caused asymptomatic persistent infection in BALB/c mice with high antibody titres and high viral loads in some organs, consistent with the above epidemiological results. This study is the first report of the isolation of EMCV in Zambia, suggesting that M. natalensis may play a role as a natural reservoir of infection.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution 4.0 International
権利情報	© Mai Kishimoto, Bernard M. Hang'ombe, William W. Hall, Yasuko Orba, Hirofumi Sawa and Michihito Sasaki, 2021. The definitive peer reviewed, edited version of this article is published in Journal of General Virology, volume102, issue3, 2021, 10.1099/jgv.0.001564.
PISSN	0022-1317
EISSN	1465-2099
NCID	AA00698722
関連情報	PMID 33533710
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1099/jgv.0.001564