E190V substitution of H6 hemagglutinin is one of key factors for binding to sulfated sialylated glycan receptor and infection to chickens

Kikutani, Yuto; Okamatsu, Masatoshi; Nishihara, Shoko; Takase, Sayaka; Hiono, Takahiro; de Vries, P. Robert; McBride, Ryan; Matsuno, Keita; Kida, Hiroshi; Sakoda, Yoshihiro

E190V substitution of H6 hemagglutinin is one of key factors for binding to sulfated sialylated glycan receptor and infection to chickens

Kikutani, Yuto; Okamatsu, Masatoshi; Nishihara, Shoko et al.

Microbiology and immunology, 2020, 64(4), 304-312

Permalink : https://hdl.handle.net/2115/81008

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論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/81008
タイトル	E190V substitution of H6 hemagglutinin is one of key factors for binding to sulfated sialylated glycan receptor and infection to chickens
著者	Kikutani, Yuto ; Okamatsu, Masatoshi ; Nishihara, Shoko ; Takase, Sayaka ; Hiono, Takahiro ; de Vries, P. Robert ; McBride, Ryan ; Matsuno, Keita ; Kida, Hiroshi ; Sakoda, Yoshihiro
著者名 Kikutani, Yuto
e-Rad_Researcher 00507163 著者名 Okamatsu, Masatoshi
著者名 Nishihara, Shoko
e-Rad_Researcher 60236221 著者名 Takase, Sayaka 著者別名 Yoden, Sayaka
e-Rad_Researcher 00775819 著者名 Hiono, Takahiro
著者名 de Vries, P. Robert
著者名 McBride, Ryan
e-Rad_Researcher 40753306 著者名 Matsuno, Keita
e-Rad_Researcher 10109506 著者名 Kida, Hiroshi
e-Rad_Researcher 40333637 著者名 Sakoda, Yoshihiro
言語	英語
キーワード	avian influenza virus
	hemagglutinin
	interspecies transmission
	sialic acid receptor
	sulfated glycans
発行日	2020-04-16
出版者	John Wiley & Sons
収録物名	Microbiology and immunology
巻(号)	64(4)
ページ	304-312
抄録	Avian influenza viruses (AIVs) recognize sialic acid linked alpha 2,3 to galactose (SA alpha 2,3Gal) glycans as receptors. In this study, the interactions between hemagglutinins (HAs) of AIVs and sulfated SA alpha 2,3Gal glycans were analyzed to clarify the molecular basis of interspecies transmission of AIVs from ducks to chickens. It was revealed that E190V and N192D substitutions of the HA increased the recovery of viruses derived from an H6 duck virus isolate, A/duck/Hong Kong/960/1980 (H6N2), in chickens. Recombinant HAs from an H6 chicken virus, A/chicken/Tainan/V156/1999 (H6N1), bound to sulfated SA alpha 2,3Gal glycans, whereas the HAs from an H6 duck virus did not. Binding preference of mutant HAs revealed that an E190V substitution is critical for the recognition of sulfated SA alpha 2,3Gal glycans. These results suggest that the binding of the HA from H6 AIVs to sulfated SA alpha 2,3Gal glycans explains a part of mechanisms of interspecies transmission of AIVs from ducks to chickens.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	This is the peer reviewed version of the following article: Kikutani Y, OkamatsuM, Nishihara S, et al. E190V substitution of H6hemagglutinin is one of key factors for binding tosulfated sialylated glycan receptor and infection tochickens.Microbiology and Immunology. 2020;1–9, which has been published in final form at https://doi.org/10.1111/1348-0421.12773. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
PISSN	0385-5600
EISSN	1348-0421
NCID	AA00738350
関連情報	PMID 31943329
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1111/1348-0421.12773