Analysis of alpha-Defensin 5 Secretion in Differentiated Caco-2 Cells : Comparison of Cell Bank Origin

Yasuda, Genki; Kobayashi, Masaki; Kubota, Atsuhito et al.

Biological and pharmaceutical bulletin, 2021, 44(2), 275-278

Permalink : https://hdl.handle.net/2115/81034

このアイテムのアクセス数:595件（2026-08-18 21:42 集計）

閲覧可能ファイル 

ファイル フォーマット サイズ 閲覧回数 説明
https://doi.org/10.1248/bpb.b20-00644     16

論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

アクセス権
open access
URI
https://hdl.handle.net/2115/81034
タイトル
Analysis of alpha-Defensin 5 Secretion in Differentiated Caco-2 Cells : Comparison of Cell Bank Origin
著者
Yasuda, Genki ; Kobayashi, Masaki ; Kubota, Atsuhito ; Narumi, Katsuya ; Furugen, Ayako ; Saito, Yoshitaka ; Satoh, Takashi ; Suzuki, Natsuko ; Iseki, Ken
著者名
Yasuda, Genki
e-Rad_Researcher
70431319
著者名
Kobayashi, Masaki
著者名
Kubota, Atsuhito
e-Rad_Researcher
90746752
著者名
Narumi, Katsuya
e-Rad_Researcher
90767261
著者名
Furugen, Ayako
e-Rad_Researcher
00835001
著者名
Saito, Yoshitaka
著者名
Satoh, Takashi
著者名
Suzuki, Natsuko
e-Rad_Researcher
40203062
著者名
Iseki, Ken
言語
英語
キーワード
α-defensin 5
Caco-2 cell
cytokine
differentiation
alkaline phosphatase
発行日
2021-02-01
出版者
The Pharmaceutical Society of Japan
日本薬学会
収録物名
Biological and pharmaceutical bulletin
巻(号)
44(2)
ページ
275-278
抄録
α-Defensin 5 has a particularly broad antibacterial spectrum; it eliminates pathogenic microorganisms and regulates intestinal flora. Although Caco-2 cells are similar to small intestinal cells, it is unclear whether they secrete α-defensin 5. Therefore, we investigated whether Caco-2 cells secrete α-defensin 5 and determined the secretion mechanism using cells from three cell banks (ATCC, DSMZ, and RIKEN). The Caco-2 cell proliferation rate increased with the number of culture days, irrespective of cell bank origin. On the other hand, the alkaline phosphatase activity, which affects cell differentiation and the mRNA levels of several cytokines, such as interleukin 8 (IL-8), IL-6, IL-1β, tumor necrosis factor-α (TNF-α), and IL-2, in the Caco-2 cells fluctuated with the number of culture days, and differed for each cell bank. α-Defensin 5 secretion was detected in all three cell bank Caco-2 cells; particularly, the ATCC Caco-2 cells grew linearly depending on the cell culture day as well as the levels of IL-8 and TNF-α mRNA. This suggested that α-defensin 5 secretion in the ATCC Caco-2 cells was associated with fluctuations in the mRNA levels of various cytokines, such as IL-8 and TNF-α. In conclusion, Caco-2 cells may be a simple model for screening health food components and drugs that affect α-defensin 5 secretion.
資源タイプ
学術雑誌論文
出版タイプ
Version of Record
PISSN
0918-6158
EISSN
1347-5215
関連情報 (isIdenticalTo)
出版社版DOI
https://doi.org/10.1248/bpb.b20-00644