Simultaneous real-time analysis of Paneth cell and intestinal stem cell response to interferon-gamma by a novel stem cell niche tracking method

Yokoi, Yuki; Adachi, Takahiro; Sugimoto, Rina; Kikuchi, Mani; Ayabe, Tokiyoshi; Nakamura, Kiminori

Simultaneous real-time analysis of Paneth cell and intestinal stem cell response to interferon-gamma by a novel stem cell niche tracking method

Yokoi, Yuki; Adachi, Takahiro; Sugimoto, Rina et al.

Biochemical and biophysical research communications, 2021, 545, 14-19

Permalink : https://hdl.handle.net/2115/81037

このアイテムのアクセス数:571件（2026-08-08 04:32 集計）

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https://doi.org/10.1016/j.bbrc.2021.01.050			21

論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/81037
タイトル	Simultaneous real-time analysis of Paneth cell and intestinal stem cell response to interferon-gamma by a novel stem cell niche tracking method
著者	Yokoi, Yuki ; Adachi, Takahiro ; Sugimoto, Rina ; Kikuchi, Mani ; Ayabe, Tokiyoshi ; Nakamura, Kiminori
著者名 Yokoi, Yuki
著者名 Adachi, Takahiro
著者名 Sugimoto, Rina
著者名 Kikuchi, Mani
著者名 Ayabe, Tokiyoshi
e-Rad_Researcher 80381276 著者名 Nakamura, Kiminori
言語	英語
キーワード	Paneth cell
	Intestinal stem cell
	Stem cell niche
	Interferon-gamma
	Live imaging
発行日	2021-03-19
出版者	Elsevier
収録物名	Biochemical and biophysical research communications
巻(号)	545
ページ	14-19
抄録	Paneth cells and Lgr5+ intestinal stem cells (Lgr5+ ISCs) constitute the stem cell niche and maintain small intestinal epithelial integrity by recognizing various niche factors derived from subepithelial cells and external antigens. Although it has been known that interferon-gamma (IFN-gamma), a Th1 cytokine, is associated with intestinal epithelial disruption during inflammation as a niche factor, dynamics of Paneth cells and Lgr5+ ISCs in response to IFN-gamma remain to be understood. Here we show that CAG-tdTomato;Lgr5-EGFP (CT-LE) mice generated in this study enable to identify Paneth cells and Lgr5+ ISCs separately by fluorescence signals. Lgr5+ ISCs underwent cell death a little earlier than Paneth cells in response to IFN-gamma by simultaneous tracking using CT-LE mice. In addition, the timing of cell death in most Paneth cells overlapped with Lgr5+ ISCs, suggesting that Paneth cell depletion is induced directly by IFN-gamma. Taken together, we established a novel simultaneous stem cell niche tracking method and clarified the involvement of both Paneth cells and Lgr5+ ISCs in stem cell niche damage induced by IFN-gamma, further contribute to understanding the mechanism for maintaining intestinal homeostasis by stem cell niche. (C) 2021 The Authors. Published by Elsevier Inc.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution 4.0 International
PISSN	0006-291X
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1016/j.bbrc.2021.01.050