Characterisation of a cysteine protease from poultry red mites and its potential use as a vaccine for chickens

Murata, Shiro; Taniguchi, Ayaka; Isezaki, Masayoshi; Fujisawa, Sotaro; Sakai, Eishi; Taneno, Akira; Ichii, Osamu; Ito, Takuya; Maekawa, Naoya; Okagawa, Tomohiro; Konnai, Satoru; Ohashi, Kazuhiko

Characterisation of a cysteine protease from poultry red mites and its potential use as a vaccine for chickens

Murata, Shiro; Taniguchi, Ayaka; Isezaki, Masayoshi et al.

Parasite, 2021, 28, 9

Permalink : https://hdl.handle.net/2115/81083

このアイテムのアクセス数:493件（2026-08-09 16:48 集計）

閲覧可能ファイル

ファイル	フォーマット	サイズ	閲覧回数	説明
https://doi.org/10.1051/parasite/2021005			13

論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

アクセス権	open access
URI	https://hdl.handle.net/2115/81083
タイトル	Characterisation of a cysteine protease from poultry red mites and its potential use as a vaccine for chickens
著者	Murata, Shiro ; Taniguchi, Ayaka ; Isezaki, Masayoshi ; Fujisawa, Sotaro ; Sakai, Eishi ; Taneno, Akira ; Ichii, Osamu ; Ito, Takuya ; Maekawa, Naoya ; Okagawa, Tomohiro ; Konnai, Satoru ; Ohashi, Kazuhiko
e-Rad_Researcher 10579163 著者名 Murata, Shiro
著者名 Taniguchi, Ayaka
著者名 Isezaki, Masayoshi
著者名 Fujisawa, Sotaro
著者名 Sakai, Eishi
著者名 Taneno, Akira
e-Rad_Researcher 60547769 著者名 Ichii, Osamu
著者名 Ito, Takuya
e-Rad_Researcher 70829035 著者名 Maekawa, Naoya
e-Rad_Researcher 80829036 著者名 Okagawa, Tomohiro
e-Rad_Researcher 40396304 著者名 Konnai, Satoru
e-Rad_Researcher 90250498 著者名 Ohashi, Kazuhiko
言語	英語
キーワード	Poultry red mite
	Cysteine protease
	Cathepsin L
	Vaccine candidate
	Deg-CPR-1
	Dermanyssus gallinae
発行日	2021-02-03
出版者	EDP Sciences
収録物名	Parasite
巻(号)	28
ページ	9
抄録	Poultry red mites (PRMs, Dermanyssus gallinae) are ectoparasites that negatively affect farmed chickens, leading to serious economic losses worldwide. Acaricides have been used to control PRMs in poultry houses. However, some PRMs have developed resistance to acaricides, and therefore different approaches are required to manage the problems caused by PRMs. Vaccination of chickens is one of the methods being considered to reduce the number of PRMs in poultry houses. In a previous study, a cysteine protease, Deg-CPR-1, was identified as a candidate vaccine against PRMs distributed in Europe. In this study, we investigated the characteristics of Deg-CPR-1. A phylogenetic analysis revealed that Deg-CPR-1 is closely related to the digestive cysteine proteases of other mite species, and it was classified into a cluster different from that of chicken cathepsins. Deg-CPR-1 of PRMs in Japan has an amino acid substitution compared with that of PRMs in Europe, but it showed efficacy as a vaccine, consistent with previous findings. Deg-CPR-1 exhibited cathepsin L-like enzyme activity. In addition, the Deg-CPR-1 mRNA was expressed in the midgut and in all stages of PRMs that feed on blood. These results imply that Deg-CPR-1 in the midgut may have important functions in physiological processes, and the inhibition of its expression may contribute to the efficacy of a Deg-CPR-1-based vaccine. Further research is required to fully understand the mechanisms of vaccine efficacy.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution 4.0 International
PISSN	1252-607X
EISSN	1776-1042
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1051/parasite/2021005