Three-dimensional visualization of mitochondria in conidia of Pyricularia oryzae using green fluorescent protein (GFP) fused with citrate synthase (CitA)

Syib'li, Muhammad Akhid; Kodama, Aoi; Abe, Ayumi et al.

Journal of general plant pathology, 2020, 86(4), 250-256

Permalink : https://hdl.handle.net/2115/81224

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アクセス権
open access
URI
https://hdl.handle.net/2115/81224
タイトル
Three-dimensional visualization of mitochondria in conidia of Pyricularia oryzae using green fluorescent protein (GFP) fused with citrate synthase (CitA)
著者
Syib'li, Muhammad Akhid ; Kodama, Aoi ; Abe, Ayumi ; Sone, Teruo
著者名
Syib'li, Muhammad Akhid
著者名
Kodama, Aoi
e-Rad_Researcher
70374626
著者名
Abe, Ayumi
e-Rad_Researcher
00333633
著者名
Sone, Teruo
言語
英語
キーワード
Pyricularia oryzae
Mitochondria
Citrate synthase (CitA)-GFP
3D visualization
発行日
2020-05-12
出版者
Springer
収録物名
Journal of general plant pathology
巻(号)
86(4)
ページ
250-256
抄録
The mitochondrion is the target organelle for QoI (quinone outside inhibitor) antifungal drugs, but mitochondrial behavior in Pyricularia oryzae remains unclear. Conidial mitochondria in fungal isolate transformed with citrate synthase (CitA)-GFP fusion gene, were observed in situ using laser-scanning confocal microscopy and computer analysis. After mycelium was harvested at 36 h and aerial mycelia removed, conidia with 1, 2, or 3 cells were observed over time. The 3D visualization of mitochondria in these conidia showed that mitochondria were initially shaped like dots and developed into a network as the conidia aged.
資源タイプ
学術雑誌論文
出版タイプ
Accepted Manuscript
権利情報
This is a post-peer-review, pre-copyedit version of an article published in Journal of general plant pathology. The final authenticated version is available online at: http://dx.doi.org/10.1007/s10327-020-00928-0
PISSN
1345-2630
関連情報 (isVersionOf)
出版社版DOI
https://doi.org/10.1007/s10327-020-00928-0