Induction of Apoptosis and Regulation of MicroRNA Expression by (2E,6E)-2,6-bis-(4-hydroxy-3-methoxybenzylidene)-cyclohexanone (BHMC) Treatment on MCF-7 Breast Cancer Cells

Yeap, Swee Keong; Mohd Ali, Norlaily; Akhtar, Muhammad Nadeem; Razak, Nursyamirah Abd; Chong, Zhi Xiong; Ho, Wan Yong; Boo, Lily; Zareen, Seema; Kurniawan, Tonni Agustiono; Avtar, Ram; Ng, Stephanie Y. L.; Ong, Alan Han Kiat; Alitheen, Noorjahan Banu

Induction of Apoptosis and Regulation of MicroRNA Expression by (2E,6E)-2,6-bis-(4-hydroxy-3-methoxybenzylidene)-cyclohexanone (BHMC) Treatment on MCF-7 Breast Cancer Cells

Yeap, Swee Keong; Mohd Ali, Norlaily; Akhtar, Muhammad Nadeem et al.

Molecules, 2021, 26(5), 1277

Permalink : https://hdl.handle.net/2115/81239

このアイテムのアクセス数:749件（2026-08-27 00:37 集計）

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アクセス権	open access
URI	https://hdl.handle.net/2115/81239
タイトル	Induction of Apoptosis and Regulation of MicroRNA Expression by (2E,6E)-2,6-bis-(4-hydroxy-3-methoxybenzylidene)-cyclohexanone (BHMC) Treatment on MCF-7 Breast Cancer Cells
著者	Yeap, Swee Keong ; Mohd Ali, Norlaily ; Akhtar, Muhammad Nadeem ; Razak, Nursyamirah Abd ; Chong, Zhi Xiong ; Ho, Wan Yong ; Boo, Lily ; Zareen, Seema ; Kurniawan, Tonni Agustiono ; Avtar, Ram ; Ng, Stephanie Y. L. ; Ong, Alan Han Kiat ; Alitheen, Noorjahan Banu
著者名 Yeap, Swee Keong
著者名 Mohd Ali, Norlaily
著者名 Akhtar, Muhammad Nadeem
著者名 Razak, Nursyamirah Abd
著者名 Chong, Zhi Xiong
著者名 Ho, Wan Yong
著者名 Boo, Lily
著者名 Zareen, Seema
著者名 Kurniawan, Tonni Agustiono
e-Rad_Researcher 90648057 著者名 Avtar, Ram
著者名 Ng, Stephanie Y. L.
著者名 Ong, Alan Han Kiat
著者名 Alitheen, Noorjahan Banu
言語	英語
キーワード	breast cancer
	2,6-bis-(4-hydroxy-3-methoxybenzylidene)-cyclohexanone (BHMC)
	MCF-7
	apoptosis
	miRNA
発行日	2021-03-01
出版者	MDPI
収録物名	Molecules
巻(号)	26(5)
ページ	1277
抄録	(2E,6E)-2,6-bis-(4-hydroxy-3-methoxybenzylidene)-cyclohexanone (BHMC) is a synthetic curcumin analogue, which has been reported to possess anti-tumor, anti-metastatic, and anti-invasion properties on estrogen receptor (ER) negative breast cancer cells in vitro and in vivo. However, the cytotoxic effects of BHMC on ER positive breast cancer cells were not widely reported. This study was aimed to investigate the cytotoxic potential of BHMC on MCF-7 cells using cell viability, cell cycle, and apoptotic assays. Besides, microarray and quantitative polymerase chain reaction (qPCR) were performed to identify the list of miRNAs and genes, which could be dysregulated following BHMC treatment. The current study discovered that BHMC exhibits selective cytotoxic effects on ER positive MCF-7 cells as compared to ER negative MDA-MB-231 cells and normal breast cells, MCF-10A. BHMC was shown to promote G2/M cell cycle arrest and apoptosis in MCF-7 cells. Microarray and qPCR analysis demonstrated that BHMC treatment would upregulate several miRNAs like miR-3195 and miR-30a-3p and downregulate miRNAs such as miR-6813-5p and miR-6132 in MCF-7 cells. Besides, BHMC administration was also found to downregulate few tumor-promoting genes like VEGF and SNAIL in MCF-7. In conclusion, BHMC induced apoptosis in the MCF-7 cells by altering the expressions of apoptotic-regulating miRNAs and associated genes.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution 4.0 International
EISSN	1420-3049
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.3390/molecules26051277