Characterization of the Ghd8 Flowering Time Gene in a Mini-Core Collection of Miscanthus sinensis

Guo, Zhihui; Xu, Meilan; Nagano, Hironori; Clark, Lindsay V.; Sacks, Erik J.; Yamada, Toshihiko

Characterization of the Ghd8 Flowering Time Gene in a Mini-Core Collection of Miscanthus sinensis

Guo, Zhihui; Xu, Meilan; Nagano, Hironori et al.

Genes, 2021, 12(2), 288

Permalink : https://hdl.handle.net/2115/81356

このアイテムのアクセス数:439件（2026-08-14 18:18 集計）

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https://doi.org/10.3390/genes12020288			29

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アクセス権	open access
URI	https://hdl.handle.net/2115/81356
タイトル	Characterization of the Ghd8 Flowering Time Gene in a Mini-Core Collection of Miscanthus sinensis
著者	Guo, Zhihui ; Xu, Meilan ; Nagano, Hironori ; Clark, Lindsay V. ; Sacks, Erik J. ; Yamada, Toshihiko
著者名 Guo, Zhihui
著者名 Xu, Meilan
著者名 Nagano, Hironori
著者名 Clark, Lindsay V.
著者名 Sacks, Erik J.
著者名 Yamada, Toshihiko
言語	英語
キーワード	Miscanthus sinensis
	Ghd8
	gene expression
	flowering time
	sequence diversity
	geographic distribution
発行日	2021-02-19
出版者	MDPI
収録物名	Genes
巻(号)	12(2)
ページ	288
抄録	The optimal flowering time for bioenergy crop Miscanthus is essential for environmental adaptability and biomass accumulation. However, little is known about how genes controlling flowering in other grasses contribute to flowering regulation in Miscanthus. Here, we report on the sequence characterization and gene expression of Miscanthus sinensis Ghd8, a transcription factor encoding a HAP3/NF-YB DNA-binding domain, which has been identified as a major quantitative trait locus in rice, with pleiotropic effects on grain yield, heading date and plant height. In M. sinensis, we identified two homoeologous loci, MsiGhd8A located on chromosome 13 and MsiGhd8B on chromosome 7, with one on each of this paleo-allotetraploid species' subgenomes. A total of 46 alleles and 28 predicted protein sequence types were identified in 12 wild-collected accessions. Several variants of MsiGhd8 showed a geographic and latitudinal distribution. Quantitative real-time PCR revealed that MsiGhd8 expressed under both long days and short days, and MsiGhd8B showed a significantly higher expression than MsiGhd8A. The comparison between flowering time and gene expression indicated that MsiGhd8B affected flowering time in response to day length for some accessions. This study provides insight into the conserved function of Ghd8 in the Poaceae, and is an important initial step in elucidating the flowering regulatory network of Miscanthus.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution 4.0 International
PISSN	2073-4425
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.3390/genes12020288