Exploring Prokaryotic and Eukaryotic Microbiomes Helps in Detecting Tick-Borne Infectious Agents in the Blood of Camels

Mohamed, Wessam Mohamed Ahmed; Ali, Alsagher O.; Mahmoud, Hassan Y. A. H.; Omar, Mosaab A.; Chatanga, Elisha; Salim, Bashir; Naguib, Doaa; Anders, Jason L.; Nonaka, Nariaki; Moustafa, Mohamed Abdallah Mohamed; Nakao, Ryo

Exploring Prokaryotic and Eukaryotic Microbiomes Helps in Detecting Tick-Borne Infectious Agents in the Blood of Camels

Mohamed, Wessam Mohamed Ahmed; Ali, Alsagher O.; Mahmoud, Hassan Y. A. H. et al.

Pathogens, 2021, 10(3), 351

Permalink : https://hdl.handle.net/2115/81491

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アクセス権	open access
URI	https://hdl.handle.net/2115/81491
タイトル	Exploring Prokaryotic and Eukaryotic Microbiomes Helps in Detecting Tick-Borne Infectious Agents in the Blood of Camels
著者	Mohamed, Wessam Mohamed Ahmed ; Ali, Alsagher O. ; Mahmoud, Hassan Y. A. H. ; Omar, Mosaab A. ; Chatanga, Elisha ; Salim, Bashir ; Naguib, Doaa ; Anders, Jason L. ; Nonaka, Nariaki ; Moustafa, Mohamed Abdallah Mohamed ; Nakao, Ryo
著者名 Mohamed, Wessam Mohamed Ahmed
著者名 Ali, Alsagher O.
著者名 Mahmoud, Hassan Y. A. H.
著者名 Omar, Mosaab A.
著者名 Chatanga, Elisha
著者名 Salim, Bashir
著者名 Naguib, Doaa
著者名 Anders, Jason L.
e-Rad_Researcher 50281853 著者名 Nonaka, Nariaki
著者名 Moustafa, Mohamed Abdallah Mohamed
e-Rad_Researcher 50633955 著者名 Nakao, Ryo
言語	英語
キーワード	Candidatus Anaplasma camelii
	eukaryotes
	microbiome
	Theileria
	Trypanosoma evansi
発行日	2021-03-16
出版者	MDPI
収録物名	Pathogens
巻(号)	10(3)
ページ	351
抄録	Dromedary camels (Camelus dromedarius) are widely distributed in Africa, the Middle East and northern India. In this study, we aimed to detect tick-borne pathogens through investigating prokaryotic and eukaryotic microorganisms in camel blood based on a metagenomic approach and then to characterize potentially pathogenic organisms using traditional molecular techniques. We showed that the bacteria circulating in the blood of camels is dominated by Proteobacteria, Bacteroidetes, Firmicutes and Actinobacteria. At the genus level, Sediminibacterium, Hydrotalea, Bradyrhizobium and Anaplasma were the most abundant taxa. Eukaryotic profile was dominated by Fungi, Charophyta and Apicomplexa. At the genus level, Theileria was detected in 10 out of 18 samples, while Sarcocystis, Hoplorhynchus and Stylocephalus were detected in one sample each. Our metagenomic approach was successful in the detection of several pathogens or potential pathogens including Anaplasma sp., Theileria ovis, Th. separata, Th. annulate, Th. mutans-like and uncharacterized Theileria sp. For further characterization, we provided the partial sequences of citrate synthase (gltA) and heat-shock protein (groEL) genes of Candidatus Anaplasma camelii. We also detected Trypanosoma evansi type A using polymerase chain reaction (PCR) targeting the internal transcribed spacer 1 (ITS1) region. This combined metagenomic and traditional approach will contribute to a better understanding of the epidemiology of pathogens including tick-borne bacteria and protozoa in animals.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.3390/pathogens10030351