Host ESCRT factors are recruited during chikungunya virus infection and are required for the intracellular viral replication cycle

Torii, Shiho; Orba, Yasuko; Sasaki, Michihito; Tabata, Koshiro; Wada, Yuji; Carr, Michael; Hobson-Peters, Jody; Hall, Roy A.; Takada, Ayato; Fukuhara, Takasuke; Matsuura, Yoshiharu; Hall, William W.; Sawa, Hirofumi

Host ESCRT factors are recruited during chikungunya virus infection and are required for the intracellular viral replication cycle

Torii, Shiho; Orba, Yasuko; Sasaki, Michihito et al.

Journal of Biological Chemistry (JBC), 2020, 295(23), 7941-7957

Permalink : https://hdl.handle.net/2115/81721

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論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/81721
タイトル	Host ESCRT factors are recruited during chikungunya virus infection and are required for the intracellular viral replication cycle
著者	Torii, Shiho ; Orba, Yasuko ; Sasaki, Michihito ; Tabata, Koshiro ; Wada, Yuji ; Carr, Michael ; Hobson-Peters, Jody ; Hall, Roy A. ; Takada, Ayato ; Fukuhara, Takasuke ; Matsuura, Yoshiharu ; Hall, William W. ; Sawa, Hirofumi
著者名 Torii, Shiho
e-Rad_Researcher 60507169 著者名 Orba, Yasuko
e-Rad_Researcher 70609403 著者名 Sasaki, Michihito
著者名 Tabata, Koshiro
著者名 Wada, Yuji
e-Rad_Researcher 70769588 著者名 Carr, Michael
著者名 Hobson-Peters, Jody
著者名 Hall, Roy A.
e-Rad_Researcher 10292062 著者名 Takada, Ayato
e-Rad_Researcher 70598739 著者名 Fukuhara, Takasuke
e-Rad_Researcher 50157252 著者名 Matsuura, Yoshiharu
著者名 Hall, William W.
e-Rad_Researcher 30292006 著者名 Sawa, Hirofumi
言語	英語
キーワード	Chikungunya virus
	endosomal sorting complexes required for transport (ESCRT)
	alphavirus
	trafficking
	viral replication
	hepatocyte growth factor-regulated tyrosine kinase substrate (HGS)
	viral release
	host factor
	siRNA screen
	particle release
	host
発行日	2020-06-05
出版者	American Society for Biochemistry and Molecular Biology (ASBMB)
収録物名	Journal of Biological Chemistry (JBC)
巻(号)	295(23)
ページ	7941-7957
抄録	Chikungunya fever is a re-emerging zoonotic disease caused by chikungunya virus (CHIKV), a member of the Alphavirus genus in the Togaviridae family. Only a few studies have reported on the host factors required for intracellular CHIKV trafficking. Here, we conducted an imaging-based siRNA screen to identify human host factors for intracellular trafficking that are involved in CHIKV infection, examined their interactions with CHIKV proteins, and investigated the contributions of these proteins to CHIKV infection. The results of the siRNA screen revealed that host endosomal sorting complexes required for transport (ESCRT) proteins are recruited during CHIKV infection. Co-immunoprecipitation analyses revealed that both structural and nonstructural CHIKV proteins interact with hepatocyte growth factor?regulated tyrosine kinase substrate (HGS), a component of the ESCRT-0 complex. We also observed that HGS co-localizes with the E2 protein of CHIKV and with dsRNA, a marker of the replicated CHIKV genome. Results from gene knockdown analyses indicated that, along with other ESCRT factors, HGS facilitates both genome replication and post-translational steps during CHIKV infection. Moreover, we show that ESCRT factors are also required for infections with other alphaviruses. We conclude that during CHIKV infection, several ESCRT factors are recruited via HGS and are involved in viral genome replication and post-translational processing of viral proteins.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	This research was originally published in Journal of Biological Chemistry. Shiho Torii, Yasuko Orba, Michihito Sasaki, Koshiro Tabata, Yuji Wada, Michael Carr, Jody Hobson-Peters, Roy A. Hall, Ayato Takada, Takasuke Fukuhara, Yoshiharu Matsuura, William W. Hall and Hirofumi Sawa. Host ESCRT factors are recruited during chikungunya virus infection and are required for the intracellular viral replication cycle. Journal of Biological Chemistry. 2020; Vol.295:7941-7957. ©the American Society for Biochemistry and Molecular Biology.
PISSN	0021-9258
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1074/jbc.RA119.012303