open access

https://hdl.handle.net/2115/81973

Preparation of beta(1 -> 3)/beta(1 -> 4) xylooligosaccharides from red alga dulse by two xylanases from Streptomyces thermogriseus

英語

Actinomycete

2021-05-12

Springer

Bioresources and bioprocessing

8(1)

38

Red alga dulse contains xylan with beta(1 -> 3)/beta(1 -> 4) linkages. We previously prepared xylooligosaccharides (XOSs) from dulse xylan; however, the product contained many D-xylose residues and fewer XOSs with beta(1 -> 3) linkages. To improve the efficiency of XOS production, we prepared two recombinant endoxylanases from Streptomyces thermogriseus (StXyl10 and StXyl11). Comparing the k(cat)/K-m values for dulse xylan, this value from StXyl10 was approximately two times higher than that from StXyl11. We then determined the suitable conditions for XOS production. As a result, dulse XOS was prepared by the successive hydrolysis of 10 mg/mL dulse xylan by 0.5 mu g/mL StXyl10 for 4 h at 50 degrees C and then 2.0 mu g/mL StXyl11 for 36 h at 60 degrees C. Xylan was converted into 95.8% XOS, including 59.7% XOS with a beta(1 -> 3) linkage and 0.97% D-xylose. Our study provides useful information for the production of XOSs with beta(1 -> 3) linkages.

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