Abundant Synthesis of Netrin-1 in Satellite Cell-Derived Myoblasts Isolated from EDL Rather Than Soleus Muscle Regulates Fast-Type Myotube Formation

Suzuki, Takahiro; Mori, Aika; Maeno, Takahiro; Arimatsu, Rio; Ichimura, Emi; Nishi, Yuriko; Hisaeda, Kouga; Yamaya, Yuki; Kobayashi, Ken; Nakamura, Mako; Tatsumi, Ryuichi; Ojima, Koichi; Nishimura, Takanori

Abundant Synthesis of Netrin-1 in Satellite Cell-Derived Myoblasts Isolated from EDL Rather Than Soleus Muscle Regulates Fast-Type Myotube Formation

Suzuki, Takahiro; Mori, Aika; Maeno, Takahiro et al.

International Journal of Molecular Sciences, 2021, 22(9), 4499

Permalink : https://hdl.handle.net/2115/81995

このアイテムのアクセス数:451件（2026-08-19 21:45 集計）

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https://doi.org/10.3390/ijms22094499			16

論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/81995
タイトル	Abundant Synthesis of Netrin-1 in Satellite Cell-Derived Myoblasts Isolated from EDL Rather Than Soleus Muscle Regulates Fast-Type Myotube Formation
著者	Suzuki, Takahiro ; Mori, Aika ; Maeno, Takahiro ; Arimatsu, Rio ; Ichimura, Emi ; Nishi, Yuriko ; Hisaeda, Kouga ; Yamaya, Yuki ; Kobayashi, Ken ; Nakamura, Mako ; Tatsumi, Ryuichi ; Ojima, Koichi ; Nishimura, Takanori
著者名 Suzuki, Takahiro
著者名 Mori, Aika
著者名 Maeno, Takahiro
著者名 Arimatsu, Rio
著者名 Ichimura, Emi
著者名 Nishi, Yuriko
著者名 Hisaeda, Kouga
著者名 Yamaya, Yuki
e-Rad_Researcher 30449003 著者名 Kobayashi, Ken
著者名 Nakamura, Mako
著者名 Tatsumi, Ryuichi
著者名 Ojima, Koichi
e-Rad_Researcher 10237729 著者名 Nishimura, Takanori
言語	英語
キーワード	satellite cells
	netrin-1
	myotube
	myofiber type
	fast-twitch
	myosin heavy chain
発行日	2021-05
出版者	MDPI
収録物名	International Journal of Molecular Sciences
巻(号)	22(9)
ページ	4499
抄録	Resident myogenic stem cells (satellite cells) are attracting attention for their novel roles in myofiber type regulation. In the myogenic differentiation phase, satellite cells from soleus muscle (slow fiber-abundant) synthesize and secrete higher levels of semaphorin 3A (Sema3A, a multifunctional modulator) than those derived from extensor digitorum longus (EDL; fast fiber-abundant), suggesting the role of Sema3A in forming slow-twitch myofibers. However, the regulatory mechanisms underlying fast-twitch myotube commitment remain unclear. Herein, we focused on netrin family members (netrin-1, -3, and -4) that compete with Sema3A in neurogenesis and osteogenesis. We examined whether netrins affect fast-twitch myotube generation by evaluating their expression in primary satellite cell cultures. Initially, netrins are upregulated during myogenic differentiation. Next, we compared the expression levels of netrins and their cell membrane receptors between soleus- and EDL-derived satellite cells; only netrin-1 showed higher expression in EDL-derived satellite cells than in soleus-derived satellite cells. We also performed netrin-1 knockdown experiments and additional experiments with recombinant netrin-1 in differentiated satellite cell-derived myoblasts. Netrin-1 knockdown in myoblasts substantially reduced fast-type myosin heavy chain (MyHC) expression; exogenous netrin-1 upregulated fast-type MyHC in satellite cells. Thus, netrin-1 synthesized in EDL-derived satellite cells may promote myofiber type commitment of fast muscles.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution 4.0 International
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.3390/ijms22094499