open access

https://doi.org/10.14943/doctoral.k14157

https://hdl.handle.net/2115/82053

アズキとダイズにおける高温活性型レトロトランスポゾンONSENの特徴とゼブラリンの効果について

英語

2020-06-30

Hokkaido University

75p

The Ty1/copia-like retrotransposon ONSEN is conserved among Brassica species,as well as in beans, including adzuki bean (Vigna angularis(Willd.) Ohwi & Ohashi)and soybean (Glycine max (L.) Merr.), which are the economically important crops in Japan. ONSEN has acquired a heat-responsive element that is recognized by plant-derived heat stress defense factors, resulting in transcribing and producing the full-length extrachromosomal DNA under conditions with elevated temperatures. DNA methylation plays an important role in regulating the activation of transposons in plants. Therefore, chemical inhibition of DNA methyltransferases has been utilized to study the effect of DNA methylation on transposon activation. To understand the effect of DNA methylation on ONSEN activation, Arabidopsisthaliana, adzuki bean, and soybean plants were treated with zebularine, which is known to be an effective chemical demethylation agent. The results showed that ONSEN transcription levels were upregulated in zebularine-treated plants. Extrachromosomal DNA of ONSEN was also accumulated in the treated plants. However, it depends on many factors such as the concentration of zebularine, the cultivation area, or the kind of plant tissue. Another factor which might involve in ONSEN transcription and transposition was chromosomal location. In soybean, most of the ONSEN copies were located in pericentromeric regions which is chromatin repressive regions.

2020-06-30

甲第14157号

博士(生命科学)

(主査) 教授 加藤 敦之, 教授 山口 淳二, 教授 藤田 知道, 准教授 伊藤 秀臣

生命科学院（生命科学専攻）

博士論文

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