open access

https://hdl.handle.net/2115/82126

Comparative profiling and comprehensive quantification of stratum corneum ceramides in humans and mice by LC/MS/MS[S]

英語

sphingolipids

2020-06

American Society for Biochemistry and Molecular Biology (ASBMB)

Journal of Lipid Research (JLR)

61(6)

884-895

Ceramides are the predominant lipids in the stratum corneum (SC) and are crucial components for normal skin barrier function. Although the composition of various ceramide classes in the human SC has been reported, that in mice is still unknown, despite mice being widely used as animal models of skin barrier function. Here, we performed LC/MS/MS analyses using recently available ceramide class standards to measure 25 classes of free ceramides and 5 classes of protein-bound ceramides from human and mouse SC. Phytosphingosine- and 6-hydroxy sphingosine-type ceramides, which both contain an additional hydroxyl group, were abundant in the human SC (35% and 45% of total ceramides, respectively). In contrast, in mice, phytosph-ingosine- and 6-hydroxy sphingosine-type ceramides were present at similar to 1% and undetectable levels, respectively, and sphingosine-type ceramides accounted for similar to 90%. In humans, ceramides containing alpha-hydroxy FA were abundant, whereas ceramides containing beta-hydroxy or omega-hydroxy FA were abundant in mice. The hydroxylated beta-carbon in beta-hydroxy ceramides was in the (R) configuration. Genetic knockout of beta-hydroxy acyl-CoA dehydratases in HAP1 cells increased beta-hydroxy ceramide levels, suggesting that beta-hydroxy acyl-CoA, an FA-elongation cycle intermediate in the ER, is a substrate for beta-hydroxy ceramide synthesis. We anticipate that our methods and findings will help to elucidate the role of each ceramide class in skin barrier formation and in the pathogenesis of skin disorders.

学術雑誌論文

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Creative Commons Attribution 4.0 International

This research was originally published in Journal of lipid research. Kawana, Momoko et al. Comparative profiling and comprehensive quantification of stratum corneum ceramides in humans and mice by LC/MS/MS. J Lipid Res. 2020; 61(6):884-895. © the American Society for Biochemistry and Molecular Biology.

0022-2275