Novel immunological approach to asses donor reactivity of transplant recipients using a humanized mouse model

Fukasaku, Yasutomo; Goto, Ryoichi; Ganchiku, Yoshikazu; Emoto, Shin; Zaitsu, Masaaki; Watanabe, Masaaki; Kawamura, Norio; Fukai, Moto; Shimamura, Tsuyoshi; Taketomi, Akinobu

Novel immunological approach to asses donor reactivity of transplant recipients using a humanized mouse model

Fukasaku, Yasutomo; Goto, Ryoichi; Ganchiku, Yoshikazu et al.

Human immunology, 2020, 81(7), 342-353

Permalink : https://hdl.handle.net/2115/82147

このアイテムのアクセス数:658件（2026-10-25 20:59 集計）

閲覧可能ファイル

ファイル	フォーマット	サイズ	閲覧回数
Hum Immunol 81_342	pdf	1.57 MB	967
Supplemental Figure 1 (Human Immunology)	pdf	71.2 KB	190
Supplemental Figure 2 (Human Immunology)	pdf	245 KB	179
supplimental tables (Human Immunology)	docx	22.0 KB	156

論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

アクセス権	open access
URI	https://hdl.handle.net/2115/82147
タイトル	Novel immunological approach to asses donor reactivity of transplant recipients using a humanized mouse model
著者	Fukasaku, Yasutomo ; Goto, Ryoichi ; Ganchiku, Yoshikazu ; Emoto, Shin ; Zaitsu, Masaaki ; Watanabe, Masaaki ; Kawamura, Norio ; Fukai, Moto ; Shimamura, Tsuyoshi ; Taketomi, Akinobu
著者名 Fukasaku, Yasutomo
e-Rad_Researcher 10645287 著者名 Goto, Ryoichi
著者名 Ganchiku, Yoshikazu
著者名 Emoto, Shin
e-Rad_Researcher 20768981 著者名 Zaitsu, Masaaki
e-Rad_Researcher 40789848 著者名 Watanabe, Masaaki
e-Rad_Researcher 20746953 著者名 Kawamura, Norio
e-Rad_Researcher 60374344 著者名 Fukai, Moto
e-Rad_Researcher 00333617 著者名 Shimamura, Tsuyoshi
e-Rad_Researcher 70363364 著者名 Taketomi, Akinobu
言語	英語
キーワード	Liver transplantation
	Alloreactivity
	Humanized mouse
発行日	2020-07
出版者	Elsevier
収録物名	Human immunology
巻(号)	81(7)
ページ	342-353
抄録	In organ transplantation, a reproducible and robust immune-monitoring assay has not been established to determine individually tailored immunosuppressants (IS). We applied humanized mice reconstituted with human (hu-) peripheral blood mononuclear cells (PBMCs) obtained from living donor liver transplant recipients to evaluate their immune status. Engraftment of 2.5 x 10(6) hu-PBMCs from healthy volunteers and recipients in the NSG mice was achieved successfully. The reconstituted lymphocytes consisted mainly of hu-CD3(+) lymphocytes with predominant CD45RA(-)CD62L(lo) T-EM and CCR6(-)CXCR3(+)CD4(+) Th1 cells in hu-PBMC-NSG mice. Interestingly, T cell allo-reactivity of hu-PBMC-NSG mice was amplified significantly compared with that of freshly isolated PBMCs (p < 0.05). Furthermore, magnified hu-T cell responses to donor antigens (Ag) were observed in 2/10 immunosuppressed recipients with multiple acute rejection (AR) experiences, suggesting that the immunological assay in hu-PBMC-NSG mice revealed hidden risks of allograft rejection by IS. Furthermore, donor Ag-specific hyporesponsiveness was maintained in recipients who had been completely weaned off IS (n = 4), despite homeostatic proliferation of hu-T cells in the hu-PBMC-NSG mice. The immunological assay in humanized mice provides a new tool to assess recipient immunity in the absence of IS and explore the underlying mechanisms to maintaining operational tolerance.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	© 2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	0198-8859
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.humimm.2020.04.007