Novel chromosomal insertions of ISEcp1-bla(CTX-M-15) and diverse antimicrobial resistance genes in Zambian clinical isolates of Enterobacter cloacae and Escherichia coli

Shawa, Misheck; Furuta, Yoshikazu; Mulenga, Gillan; Mubanga, Maron; Mulenga, Evans; Zorigt, Tuvshinzaya; Kaile, Christone; Simbotwe, Manyando; Paudel, Atmika; Hang'ombe, Bernard; Higashi, Hideaki

Novel chromosomal insertions of ISEcp1-bla(CTX-M-15) and diverse antimicrobial resistance genes in Zambian clinical isolates of Enterobacter cloacae and Escherichia coli

Shawa, Misheck; Furuta, Yoshikazu; Mulenga, Gillan et al.

Antimicrobial resistance and infection control, 2021, 10(1), 79

Permalink : https://hdl.handle.net/2115/82151

このアイテムのアクセス数:496件（2026-08-27 13:06 集計）

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アクセス権	open access
URI	https://hdl.handle.net/2115/82151
タイトル	Novel chromosomal insertions of ISEcp1-bla(CTX-M-15) and diverse antimicrobial resistance genes in Zambian clinical isolates of Enterobacter cloacae and Escherichia coli
著者	Shawa, Misheck ; Furuta, Yoshikazu ; Mulenga, Gillan ; Mubanga, Maron ; Mulenga, Evans ; Zorigt, Tuvshinzaya ; Kaile, Christone ; Simbotwe, Manyando ; Paudel, Atmika ; Hang'ombe, Bernard ; Higashi, Hideaki
著者名 Shawa, Misheck
著者名 Furuta, Yoshikazu
著者名 Mulenga, Gillan
著者名 Mubanga, Maron
著者名 Mulenga, Evans
著者名 Zorigt, Tuvshinzaya
著者名 Kaile, Christone
著者名 Simbotwe, Manyando
e-Rad_Researcher 80832774 著者名 Paudel, Atmika
著者名 Hang'ombe, Bernard
e-Rad_Researcher 20311227 著者名 Higashi, Hideaki
言語	英語
キーワード	ISEcp1
	bla(CTX-M)
	Chromosomal insertion
	Extended spectrum beta-lactamase
	Zambia
	AMR
	Enterobacter cloacae
	Escherichia coli
発行日	2021-05-10
出版者	BioMed Central
収録物名	Antimicrobial resistance and infection control
巻(号)	10(1)
ページ	79
抄録	Background: The epidemiology of extended-spectrum beta-lactamases (ESBLs) has undergone dramatic changes, with CTX-M-type enzymes prevailing over other types. bla(CTX-M) genes, encoding CTX-M-type ESBLs, are usually found on plasmids, but chromosomal location is becoming common. Given that bla(CTX-M)-harboring strains often exhibit multidrug resistance (MDR), it is important to investigate the association between chromosomally integrated bla(CTX-M) and the presence of additional antimicrobial resistance (AMR) genes, and to identify other relevant genetic elements. Methods: A total of 46 clinical isolates of cefotaxime-resistant Enterobacteriaceae (1 Enterobacter cloacae, 9 Klebsiella pneumoniae, and 36 Escherichia coli) from Zambia were subjected to whole-genome sequencing (WGS) using MiSeq and MinION. By reconstructing nearly complete genomes, bla(CTX-M) genes were categorized as either chromosomal or plasmid-borne. Results: WGS-based genotyping identified 58 AMR genes, including four bla(CTX-M) alleles (i.e., bla(CTX-M-14), bla(CTX-M-15), bla(CTX-M-27), and bla(CTX-M-55)). Hierarchical clustering using selected phenotypic and genotypic characteristics suggested clonal dissemination of bla(CTX-M) genes. Out of 45 bla(CTX-M) gene-carrying strains, 7 harbored the gene in their chromosome. In one E. cloacae and three E. coli strains, chromosomal bla(CTX-M-15) was located on insertions longer than 10 kb. These insertions were bounded by ISEcp1 at one end, exhibited a high degree of nucleotide sequence homology with previously reported plasmids, and carried multiple AMR genes that corresponded with phenotypic AMR profiles. Conclusion: Our study revealed the co-occurrence of ISEcp1-bla(CTX-M-15) and multiple AMR genes on chromosomal insertions in E. cloacae and E. coli, suggesting that ISEcp1 may be responsible for the transposition of diverse AMR genes from plasmids to chromosomes. Stable retention of such insertions in chromosomes may facilitate the successful propagation of MDR clones among these Enterobacteriaceae species.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution 4.0 International
PISSN	2047-2994
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1186/s13756-021-00941-8