Loss of H3K27 trimethylation is frequent in IDH1-R132H but not in non-canonical IDH1/2 mutated and 1p/19q codeleted oligodendroglioma: a Japanese cohort study

Habiba, Umma; Sugino, Hirokazu; Yordanova, Roumyana et al.

Acta Neuropathologica Communications, 2021, 9(1), 95

Permalink : https://hdl.handle.net/2115/82230

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https://doi.org/10.1186/s40478-021-01194-7     16

論文情報

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アクセス権
open access
URI
https://hdl.handle.net/2115/82230
タイトル
Loss of H3K27 trimethylation is frequent in IDH1-R132H but not in non-canonical IDH1/2 mutated and 1p/19q codeleted oligodendroglioma: a Japanese cohort study
著者
Habiba, Umma ; Sugino, Hirokazu ; Yordanova, Roumyana ; Ise, Koki ; Tanei, Zen-ichi ; Ishida, Yusuke ; Tanikawa, Satoshi ; Terasaka, Shunsuke ; Sato, Ken-ichi ; Kamoshima, Yuuta ; Katoh, Masahiko ; Nagane, Motoo ; Shibahara, Junji ; Tsuda, Masumi ; Tanaka, Shinya
著者名
Habiba, Umma
著者名
Sugino, Hirokazu
著者名
Yordanova, Roumyana
著者名
Ise, Koki
著者名
Tanei, Zen-ichi
著者名
Ishida, Yusuke
著者名
Tanikawa, Satoshi
著者名
Terasaka, Shunsuke
著者名
Sato, Ken-ichi
著者名
Kamoshima, Yuuta
著者名
Katoh, Masahiko
著者名
Nagane, Motoo
著者名
Shibahara, Junji
著者名
Tsuda, Masumi
e-Rad_Researcher
70261287
著者名
Tanaka, Shinya
言語
英語
キーワード
Mutation
Wild type
Trimethylation at lysine 27 of histone 3
Glioblastoma
発行日
2021-05-21
出版者
BioMed Central
収録物名
Acta Neuropathologica Communications
巻(号)
9(1)
ページ
95
抄録
Oligodendrogliomas are defined by mutation in isocitrate dehydrogenase (NADP(+)) (IDH)1/2 genes and chromosome 1p/19q codeletion. World Health Organisation diagnosis endorses testing for 1p/19q codeletion to distinguish IDH mutant (Mut) oligodendrogliomas from astrocytomas because these gliomas require different treatments and they have different outcomes. Several methods have been used to identify 1p/19q status; however, these techniques are not routinely available and require substantial infrastructure investment. Two recent studies reported reduced immunostaining for trimethylation at lysine 27 on histone H3 (H3K27me3) in IDH Mut 1p/19q codeleted oligodendroglioma. However, the specificity of H3K27me3 immunostaining in this setting is controversial. Therefore, we developed an easy-to-implement immunohistochemical surrogate for IDH Mut glioma subclassification and evaluated a validated adult glioma cohort. We screened 145 adult glioma cases, consisting of 45 IDH Mut and 1p/19q codeleted oligodendrogliomas, 30 IDH Mut astrocytomas, 16 IDH wild-type (Wt) astrocytomas, and 54 IDH Wt glioblastomas (GBMs). We compared immunostaining with DNA sequencing and fluorescent in situ hybridization analysis and assessed differences in H3K27me3 staining between oligodendroglial and astrocytic lineages and between IDH1-R132H and non-canonical (non-R132H) IDH1/2 Mut oligodendroglioma. A loss of H3K27me3 was observed in 36/40 (90%) of IDH1-R132H Mut oligodendroglioma. In contrast, loss of H3K27me3 was never seen in IDH1-R132L or IDH2-mutated 1p/19q codeleted oligodendrogliomas. IDH Mut astrocytoma, IDH Wt astrocytoma and GBM showed preserved nuclear staining in 87%, 94%, and 91% of cases, respectively. A high recursive partitioning model predicted probability score (0.9835) indicated that the loss of H3K27me3 is frequent to IDH1-R132H Mut oligodendroglioma. Our results demonstrate H3K27me3 immunohistochemical evaluation to be a cost-effective and reliable method for defining 1p/19q codeletion along with IDH1-R132H and ATRX immunostaining, even in the absence of 1p/19q testing.
資源タイプ
学術雑誌論文
出版タイプ
Version of Record
PISSN
2051-5960
関連情報 (isIdenticalTo)
出版社版DOI
https://doi.org/10.1186/s40478-021-01194-7