DNA methylation landscape of 16 canine somatic tissues by methylation-sensitive restriction enzyme-based next generation sequencing

Yamazaki, Jumpei; Matsumoto, Yuki; Jelinek, Jaroslav; Ishizaki, Teita; Maeda, Shingo; Watanabe, Kei; Ishihara, Genki; Yamagishi, Junya; Takiguchi, Mitsuyoshi

DNA methylation landscape of 16 canine somatic tissues by methylation-sensitive restriction enzyme-based next generation sequencing

Yamazaki, Jumpei; Matsumoto, Yuki; Jelinek, Jaroslav et al.

Scientific reports, 2021, 11(1), 10005

Permalink : https://hdl.handle.net/2115/82263

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https://doi.org/10.1038/s41598-021-89279-0			11

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アクセス権	open access
URI	https://hdl.handle.net/2115/82263
タイトル	DNA methylation landscape of 16 canine somatic tissues by methylation-sensitive restriction enzyme-based next generation sequencing
著者	Yamazaki, Jumpei ; Matsumoto, Yuki ; Jelinek, Jaroslav ; Ishizaki, Teita ; Maeda, Shingo ; Watanabe, Kei ; Ishihara, Genki ; Yamagishi, Junya ; Takiguchi, Mitsuyoshi
e-Rad_Researcher 20732902 著者名 Yamazaki, Jumpei
著者名 Matsumoto, Yuki
著者名 Jelinek, Jaroslav
著者名 Ishizaki, Teita
著者名 Maeda, Shingo
著者名 Watanabe, Kei
著者名 Ishihara, Genki
e-Rad_Researcher 80535328 著者名 Yamagishi, Junya
e-Rad_Researcher 70261336 著者名 Takiguchi, Mitsuyoshi
言語	英語
発行日	2021-05-11
出版者	Nature Research
収録物名	Scientific reports
巻(号)	11(1)
ページ	10005
抄録	DNA methylation plays important functions in gene expression regulation that is involved in individual development and various diseases. DNA methylation has been well studied in human and model organisms, but only limited data exist in companion animals like dog. Using methylation-sensitive restriction enzyme-based next generation sequencing (Canine DREAM), we obtained canine DNA methylation maps of 16 somatic tissues from two dogs. In total, we evaluated 130,861 CpG sites. The majority of CpG sites were either highly methylated (>70%, 52.5-64.6% of all CpG sites analyzed) or unmethylated (<30%, 22.5-28.0% of all CpG sites analyzed) which are methylation patterns similar to other species. The overall methylation status of CpG sites across the 32 methylomes were remarkably similar. However, the tissue types were clearly defined by principle component analysis and hierarchical clustering analysis with DNA methylome. We found 6416 CpG sites located closely at promoter region of genes and inverse correlation between DNA methylation and gene expression of these genes. Our study provides basic dataset for DNA methylation profiles in dogs.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
PISSN	2045-2322
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1038/s41598-021-89279-0