Osmotic gradients induce stable dome morphogenesis on extracellular matrix

Ishida-Ishihara, Sumire; Akiyama, Masakazu; Furusawa, Kazuya; Naguro, Isao; Ryuno, Hiroki; Sushida, Takamichi; Ishihara, Seiichiro; Haga, Hisashi

Osmotic gradients induce stable dome morphogenesis on extracellular matrix

Ishida-Ishihara, Sumire; Akiyama, Masakazu; Furusawa, Kazuya et al.

Journal of Cell Science, 2020, 133(14), jcs243865

Permalink : https://hdl.handle.net/2115/82291

このアイテムのアクセス数:621件（2026-10-13 13:33 集計）

閲覧可能ファイル

ファイル	フォーマット	サイズ	閲覧回数	説明
J Cell Sci 133 -14_jcs243865	pdf	5.59 MB	627

論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

アクセス権	open access
URI	https://hdl.handle.net/2115/82291
タイトル	Osmotic gradients induce stable dome morphogenesis on extracellular matrix
著者	Ishida-Ishihara, Sumire ; Akiyama, Masakazu ; Furusawa, Kazuya ; Naguro, Isao ; Ryuno, Hiroki ; Sushida, Takamichi ; Ishihara, Seiichiro ; Haga, Hisashi
著者名 Ishida-Ishihara, Sumire
著者名 Akiyama, Masakazu
著者名 Furusawa, Kazuya
著者名 Naguro, Isao
著者名 Ryuno, Hiroki
著者名 Sushida, Takamichi
著者名 Ishihara, Seiichiro
e-Rad_Researcher 00292045 著者名 Haga, Hisashi
言語	英語
キーワード	Osmotic gradient
	Epithelial cells
	ECM
	Swelling
	Aquaporin
	Numerical simulation
発行日	2020-07
出版者	Company of Biologists
収録物名	Journal of Cell Science
巻(号)	133(14)
ページ	jcs243865
抄録	One of the fundamental processes in morphogenesis is dome formation, but many of the mechanisms involved are unexplored. Previous in vitro studies showed that an osmotic gradient is the driving factor of dome formation. However, these investigations were performed without extracellular matrix (ECM), which provides structural support to morphogenesis. With the use of ECM, we observed that basal hypertonic stress induced stable domes in vitro that have not been seen in previous studies. These domes developed as a result of ECM swelling via aquaporin water transport activity. Based on computer simulation, uneven swelling, with a positive feedback between cell stretching and enhanced water transport, was a cause of dome formation. These results indicate that osmotic gradients induce dome morphogenesis via both enhanced water transport activity and subsequent ECM swelling.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
PISSN	0021-9533
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1242/jcs.243865