Purification of Crimean-Congo hemorrhagic fever virus nucleoprotein and its utility for serological diagnosis

Lombe, Boniface Pongombo; Miyamoto, Hiroko; Saito, Takeshi; Yoshida, Reiko; Manzoor, Rashid; Kajihara, Masahiro; Shimojima, Masayuki; Fukushi, Shuetsu; Morikawa, Shigeru; Yoshikawa, Tomoki; Kurosu, Takeshi; Saijo, Masayuki; Tang, Qing; Masumu, Justin; Hawman, David; Feldmann, Heinz; Takada, Ayato

Purification of Crimean-Congo hemorrhagic fever virus nucleoprotein and its utility for serological diagnosis

Lombe, Boniface Pongombo; Miyamoto, Hiroko; Saito, Takeshi et al.

Scientific reports, 2021, 11(1), 2324

Permalink : https://hdl.handle.net/2115/82393

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アクセス権	open access
URI	https://hdl.handle.net/2115/82393
タイトル	Purification of Crimean-Congo hemorrhagic fever virus nucleoprotein and its utility for serological diagnosis
著者	Lombe, Boniface Pongombo ; Miyamoto, Hiroko ; Saito, Takeshi ; Yoshida, Reiko ; Manzoor, Rashid ; Kajihara, Masahiro ; Shimojima, Masayuki ; Fukushi, Shuetsu ; Morikawa, Shigeru ; Yoshikawa, Tomoki ; Kurosu, Takeshi ; Saijo, Masayuki ; Tang, Qing ; Masumu, Justin ; Hawman, David ; Feldmann, Heinz ; Takada, Ayato
著者名 Lombe, Boniface Pongombo
著者名 Miyamoto, Hiroko
著者名 Saito, Takeshi
e-Rad_Researcher 80435966 著者名 Yoshida, Reiko
著者名 Manzoor, Rashid
e-Rad_Researcher 70711894 著者名 Kajihara, Masahiro
e-Rad_Researcher 10422411 著者名 Shimojima, Masayuki
e-Rad_Researcher 80373398 著者名 Fukushi, Shuetsu
e-Rad_Researcher 00167686 著者名 Morikawa, Shigeru
e-Rad_Researcher 20399463 著者名 Yoshikawa, Tomoki
e-Rad_Researcher 70432432 著者名 Kurosu, Takeshi
e-Rad_Researcher 50300926 著者名 Saijo, Masayuki
著者名 Tang, Qing
著者名 Masumu, Justin
著者名 Hawman, David
著者名 Feldmann, Heinz
e-Rad_Researcher 10292062 著者名 Takada, Ayato
言語	英語
発行日	2021-01-27
出版者	Nature Research
収録物名	Scientific reports
巻(号)	11(1)
ページ	2324
抄録	Crimean-Congo hemorrhagic fever virus (CCHFV) causes a zoonotic disease, Crimean-Congo hemorrhagic fever (CCHF) endemic in Africa, Asia, the Middle East, and Southeastern Europe. However, the prevalence of CCHF is not monitored in most of the endemic countries due to limited availability of diagnostic assays and biosafety regulations required for handling infectious CCHFV. In this study, we established a protocol to purify the recombinant CCHFV nucleoprotein (NP), which is antigenically highly conserved among multiple lineages/clades of CCHFVs and investigated its utility in an enzyme-linked immunosorbent assay (ELISA) to detect CCHFV-specific antibodies. The NP gene was cloned into the pCAGGS mammalian expression plasmid and human embryonic kidney 293 T cells were transfected with the plasmid. The expressed NP molecule was purified from the cell lysate using cesium-chloride gradient centrifugation. Purified NP was used as the antigen for the ELISA to detect anti-CCHFV IgG. Using the CCHFV NP-based ELISA, we efficiently detected CCHFV-specific IgG in anti-NP rabbit antiserum and CCHFV-infected monkey serum. When compared to the commercially available Blackbox CCHFV IgG ELISA kit, our assay showed equivalent performance in detecting CCHFV-specific IgG in human sera. These results demonstrate the usefulness of our CCHFV NP-based ELISA for seroepidemiological studies.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
PISSN	2045-2322
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1038/s41598-021-81752-0