PenA, a penicillin-binding protein-type thioesterase specialized for small peptide cyclization

Matsuda, Kenichi; Fujita, Kei; Wakimoto, Toshiyuki

Journal of industrial microbiology and biotechnology, 2021, 48(3-4), kuab023

Permalink : https://hdl.handle.net/2115/82462

このアイテムのアクセス数:502件（2026-06-06 12:05 集計）

ファイル	フォーマット	サイズ	閲覧回数	説明
https://doi.org/10.1093/jimb/kuab023			16

アクセス権	open access
URI	https://hdl.handle.net/2115/82462
タイトル	PenA, a penicillin-binding protein-type thioesterase specialized for small peptide cyclization
著者	Matsuda, Kenichi ; Fujita, Kei ; Wakimoto, Toshiyuki
e-Rad_Researcher 50812301 著者名 Matsuda, Kenichi
著者名 Fujita, Kei
e-Rad_Researcher 70363900 著者名 Wakimoto, Toshiyuki
言語	英語
キーワード	Cyclopeptide
	Biocatalyst
	Cyclase
発行日	2021-03-13
出版者	Oxford University Press
収録物名	Journal of industrial microbiology and biotechnology
巻(号)	48(3-4)
ページ	kuab023
抄録	Penicillin-binding protein-type thioesterases (PBP-type TEs) are a recently identified group of peptide cyclases that catalyze head-to-tail macrolactamization of nonribosomal peptides. PenA, a new member of this group, is involved in the biosyntheses of cyclic pentapeptides. In this study, we demonstrated the enzymatic activity of PenA in vitro, and analyzed its substrate scope with a series of synthetic substrates. A comparison of the reaction profiles between PenA and SurE, a representative PBP-type TE, showed that PenA is more specialized for small peptide cyclization. A computational model provided a possible structural rationale for the altered specificity for substrate chain lengths.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
PISSN	1367-5435
EISSN	1476-5535
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1093/jimb/kuab023