Pharmacological Alteration of Cellular Mechanical Properties in Pulmonary Arterial Smooth Muscle Cells of Idiopathic Pulmonary Arterial Hypertension

Katsuragi, Shinichi; Tatsumi, Nao; Matsumoto, Mizuki; Narita, Jun; Ishii, Ryo; Suginobe, Hidehiro; Tsuru, Hirofumi; Wang, Renjie; Kogaki, Shigetoyo; Tanaka, Ryosuke; Ozono, Keiichi; Okajima, Takaharu; Ishida, Hidekazu

Pharmacological Alteration of Cellular Mechanical Properties in Pulmonary Arterial Smooth Muscle Cells of Idiopathic Pulmonary Arterial Hypertension

Katsuragi, Shinichi; Tatsumi, Nao; Matsumoto, Mizuki et al.

Cardiology Research, 2021, 12(4), 231-237

Permalink : https://hdl.handle.net/2115/82468

このアイテムのアクセス数:468件（2026-10-07 09:54 集計）

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https://doi.org/10.14740/cr1282			15

論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/82468
タイトル	Pharmacological Alteration of Cellular Mechanical Properties in Pulmonary Arterial Smooth Muscle Cells of Idiopathic Pulmonary Arterial Hypertension
著者	Katsuragi, Shinichi ; Tatsumi, Nao ; Matsumoto, Mizuki ; Narita, Jun ; Ishii, Ryo ; Suginobe, Hidehiro ; Tsuru, Hirofumi ; Wang, Renjie ; Kogaki, Shigetoyo ; Tanaka, Ryosuke ; Ozono, Keiichi ; Okajima, Takaharu ; Ishida, Hidekazu
e-Rad_Researcher 40774261 著者名 Katsuragi, Shinichi
著者名 Tatsumi, Nao
著者名 Matsumoto, Mizuki
e-Rad_Researcher 70467562 著者名 Narita, Jun
著者名 Ishii, Ryo
e-Rad_Researcher 30791595 著者名 Suginobe, Hidehiro
著者名 Tsuru, Hirofumi
著者名 Wang, Renjie
e-Rad_Researcher 00311754 著者名 Kogaki, Shigetoyo
著者名 Tanaka, Ryosuke
e-Rad_Researcher 20270770 著者名 Ozono, Keiichi
e-Rad_Researcher 70280998 著者名 Okajima, Takaharu
e-Rad_Researcher 50467552 著者名 Ishida, Hidekazu
言語	英語
キーワード	Pulmonary arterial hypertension
	Atomic force microscopy
	Smooth muscle cells
	Cellular mechanical properties
	Elasticity
	Fluidity
発行日	2021-08
出版者	Elmer Press
収録物名	Cardiology Research
巻(号)	12(4)
ページ	231-237
抄録	Background: Idiopathic pulmonary arterial hypertension (IPAH) is a progressive disease caused by vascular remodeling of the pulmonary arteries with elevated pulmonary vascular resistance. Recently, various pulmonary vasodilator drugs have become available in the clinical field, and have dramatically ameliorated the prognosis of IPAH. However, little is known about how the mechanical properties of pulmonary arterial smooth muscle cells (PASMCs) are altered under drug supplementation. Methods: Atomic force microscopy (AFM) was used to investigate the mechanical properties of PASMCs derived from a patient with IPAH (PAH-PASMCs) and a healthy control (N-PASMCs) which received the supplementation of clinically used drugs for IPAH: sildenafil, macitentan, and riociguat. Results: PASMCs derived from PAH-PASMCs were stiffer than those derived from N-PASMCs. With sildenafil treatment, the apparent Young's modulus (E-0) of cells significantly decreased in PAH-PASMCs but remained unchanged in N-PASMCs. The decrease in E-0 of PAH-PASMCs was also observed in macitentan and riociguat treatment. The stress relaxation AFM revealed that the decrease in E-0 of PAH-PASMCs resulted from a decrease in the cell elastic modulus and/or increase in cell fluidity. The combination treatment of macitentan and riociguat showed an additive effect on cell mechanical properties, implying that this clinically accepted combination therapy for IPAH influences the intracellular mechanical components. Conclusions: Pulmonary vasodilator drugs affect the mechanical properties of PAH-PASMCs, and there exists a mechanical effect of combination treatment on PAH-PASMCs.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
PISSN	1923-2829
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.14740/cr1282