Expression of Vascular Endothelial Growth Factor-C in the Trabecular Meshwork of Patients with Neovascular Glaucoma and Primary Open-Angle Glaucoma

Hase, Keitaro; Kase, Satoru; Kanda, Atsuhiro; Shinmei, Yasuhiro; Noda, Kousuke; Ishida, Susumu

Expression of Vascular Endothelial Growth Factor-C in the Trabecular Meshwork of Patients with Neovascular Glaucoma and Primary Open-Angle Glaucoma

Hase, Keitaro; Kase, Satoru; Kanda, Atsuhiro et al.

Journal of clinical medicine, 2021, 10(13), 2977

Permalink : https://hdl.handle.net/2115/82529

このアイテムのアクセス数:623件（2026-08-10 17:41 集計）

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ファイル	フォーマット	サイズ	閲覧回数	説明
https://doi.org/10.3390/jcm10132977			28

論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/82529
タイトル	Expression of Vascular Endothelial Growth Factor-C in the Trabecular Meshwork of Patients with Neovascular Glaucoma and Primary Open-Angle Glaucoma
著者	Hase, Keitaro ; Kase, Satoru ; Kanda, Atsuhiro ; Shinmei, Yasuhiro ; Noda, Kousuke ; Ishida, Susumu
著者名 Hase, Keitaro
e-Rad_Researcher 60374394 著者名 Kase, Satoru
著者名 Kanda, Atsuhiro
著者名 Shinmei, Yasuhiro
著者名 Noda, Kousuke
著者名 Ishida, Susumu
言語	英語
キーワード	VEGF-C
	VEGFR3
	trabecular meshwork
	neovascular glaucoma
	primary open-angle glaucoma
発行日	2021-07
出版者	MDPI
収録物名	Journal of clinical medicine
巻(号)	10(13)
ページ	2977
抄録	The Abstract: Purpose To investigate the expression of vascular endothelial growth factor (VEGF)-C and vascular endothelial growth factor receptor (VEGFR)3 in the trabecular meshwork (TM) of patients with glaucoma and cultured TM cells. Methods: The expressions of VEGF-C in angle tissues collected by trabeculectomy from patients with glaucoma and non-glaucomatous choroidal malignant melanoma were analyzed by immunohistochemistry. Additionally, VEGF-C concentrations were determined in the aqueous humor of patients with glaucoma by ELISA. The expressions of VEGFR3, which is a receptor of VEGF-C in cultured TM cells, were analyzed by Western blot analysis and immunocytochemistry. Cultured TM cells were stimulated by oxidative stress, hypoxia, or high glucose conditions, and VEGF-C concentrations in supernatants and cell lysates were determined by ELISA. Results: VEGF-C immunoreactivity was positive in TM tissues of glaucoma patients, but not in those of non-glaucomatous controls. VEGF-C concentrations in the aqueous humor of patients with neovascular glaucoma and primary open-angle glaucoma were lower than those with non-glaucoma patients. VEGFR3 was expressed in cultured TM cells. VEGF-C concentrations in supernatants or cell lysates of TM cells cultured under oxidative stress and hypoxia were significantly elevated compared with those under steady conditions (p < 0.05). VEGF-C concentrations in supernatants and cell lysates of TM cells cultured in high glucose were significantly higher than those in low glucose (p < 0.01). Conclusions: VEGF-C was expressed in TM tissues of patients with glaucoma, which was secreted from cultured TM cells under various pathological conditions. These results suggest that VEGF-C may be involved in the pathology of glaucoma.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.3390/jcm10132977