DsRNA Sequencing for RNA Virus Surveillance Using Human Clinical Samples

Izumi, Takuma; Morioka, Yuhei; Urayama, Syun-ichi; Motooka, Daisuke; Tamura, Tomokazu; Kawagishi, Takahiro; Kanai, Yuta; Kobayashi, Takeshi; Ono, Chikako; Morinaga, Akinari; Tomiyama, Takahiro; Iseda, Norifumi; Kosai, Yukiko; Inokuchi, Shoichi; Nakamura, Shota; Tanaka, Tomohisa; Moriishi, Kohji; Kariwa, Hiroaki; Yoshizumi, Tomoharu; Mori, Masaki; Matsuura, Yoshiharu; Fukuhara, Takasuke

DsRNA Sequencing for RNA Virus Surveillance Using Human Clinical Samples

Izumi, Takuma; Morioka, Yuhei; Urayama, Syun-ichi et al.

Viruses-Basel, 2021, 13(7), 1310

Permalink : https://hdl.handle.net/2115/82551

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アクセス権	open access
URI	https://hdl.handle.net/2115/82551
タイトル	DsRNA Sequencing for RNA Virus Surveillance Using Human Clinical Samples
著者	Izumi, Takuma ; Morioka, Yuhei ; Urayama, Syun-ichi ; Motooka, Daisuke ; Tamura, Tomokazu ; Kawagishi, Takahiro ; Kanai, Yuta ; Kobayashi, Takeshi ; Ono, Chikako ; Morinaga, Akinari ; Tomiyama, Takahiro ; Iseda, Norifumi ; Kosai, Yukiko ; Inokuchi, Shoichi ; Nakamura, Shota ; Tanaka, Tomohisa ; Moriishi, Kohji ; Kariwa, Hiroaki ; Yoshizumi, Tomoharu ; Mori, Masaki ; Matsuura, Yoshiharu ; Fukuhara, Takasuke
著者名 Izumi, Takuma
著者名 Morioka, Yuhei
著者名 Urayama, Syun-ichi
著者名 Motooka, Daisuke
著者名 Tamura, Tomokazu
著者名 Kawagishi, Takahiro
著者名 Kanai, Yuta
著者名 Kobayashi, Takeshi
著者名 Ono, Chikako
著者名 Morinaga, Akinari
著者名 Tomiyama, Takahiro
著者名 Iseda, Norifumi
著者名 Kosai, Yukiko
著者名 Inokuchi, Shoichi
著者名 Nakamura, Shota
著者名 Tanaka, Tomohisa
著者名 Moriishi, Kohji
著者名 Kariwa, Hiroaki
著者名 Yoshizumi, Tomoharu
著者名 Mori, Masaki
著者名 Matsuura, Yoshiharu
e-Rad_Researcher 70598739 著者名 Fukuhara, Takasuke
言語	英語
キーワード	RNA virus
	dsRNA
	liver transplantation
	RNA sequencing
発行日	2021-07
出版者	MDPI
収録物名	Viruses-Basel
巻(号)	13(7)
ページ	1310
抄録	Although viruses infect various organs and are associated with diseases, there may be many unidentified pathogenic viruses. The recent development of next-generation sequencing technologies has facilitated the establishment of an environmental viral metagenomic approach targeting the intracellular viral genome. However, an efficient method for the detection of a viral genome derived from an RNA virus in animal or human samples has not been established. Here, we established a method for the efficient detection of RNA viruses in human clinical samples. We then tested the efficiency of the method compared to other conventional methods by using tissue samples collected from 57 recipients of living donor liver transplantations performed between June 2017 and February 2019 at Kyushu University Hospital. The viral read ratio in human clinical samples was higher by the new method than by the other conventional methods. In addition, the new method correctly identified viral RNA from liver tissues infected with hepatitis C virus. This new technique will be an effective tool for intracellular RNA virus surveillance in human clinical samples and may be useful for the detection of new RNA viruses associated with diseases.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.3390/v13071310