Programmed death-ligand 1 expression in swine chronic infections and enhancement of interleukin-2 production via programmed death-1/programmed death-ligand 1 blockade

Ganbaatar, Otgontuya; Konnai, Satoru; Okagawa, Tomohiro; Nojima, Yutaro; Maekawa, Naoya; Ichikawa, Yoshiki; Kobayashi, Atsushi; Shibahara, Tomoyuki; Yanagawa, Yojiro; Higuchi, Hidetoshi; Kato, Yukinari; Suzuki, Yasuhiko; Murata, Shiro; Ohashi, Kazuhiko

Programmed death-ligand 1 expression in swine chronic infections and enhancement of interleukin-2 production via programmed death-1/programmed death-ligand 1 blockade

Ganbaatar, Otgontuya; Konnai, Satoru; Okagawa, Tomohiro et al.

Immunity, inflammation and disease, 2021, 9(4), 1573-1583

Permalink : https://hdl.handle.net/2115/82666

このアイテムのアクセス数:514件（2026-05-14 05:16 集計）

閲覧可能ファイル

ファイル	フォーマット	サイズ	閲覧回数	説明
https://doi.org/10.1002/iid3.510			25

論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

アクセス権	open access
URI	https://hdl.handle.net/2115/82666
タイトル	Programmed death-ligand 1 expression in swine chronic infections and enhancement of interleukin-2 production via programmed death-1/programmed death-ligand 1 blockade
著者	Ganbaatar, Otgontuya ; Konnai, Satoru ; Okagawa, Tomohiro ; Nojima, Yutaro ; Maekawa, Naoya ; Ichikawa, Yoshiki ; Kobayashi, Atsushi ; Shibahara, Tomoyuki ; Yanagawa, Yojiro ; Higuchi, Hidetoshi ; Kato, Yukinari ; Suzuki, Yasuhiko ; Murata, Shiro ; Ohashi, Kazuhiko
著者名 Ganbaatar, Otgontuya
e-Rad_Researcher 40396304 著者名 Konnai, Satoru
e-Rad_Researcher 80829036 著者名 Okagawa, Tomohiro
著者名 Nojima, Yutaro
e-Rad_Researcher 70829035 著者名 Maekawa, Naoya
著者名 Ichikawa, Yoshiki
著者名 Kobayashi, Atsushi
著者名 Shibahara, Tomoyuki
e-Rad_Researcher 20609656 著者名 Yanagawa, Yojiro
著者名 Higuchi, Hidetoshi
著者名 Kato, Yukinari
e-Rad_Researcher 90206540 著者名 Suzuki, Yasuhiko
e-Rad_Researcher 10579163 著者名 Murata, Shiro
e-Rad_Researcher 90250498 著者名 Ohashi, Kazuhiko
言語	英語
キーワード	interleukin-2
	PD-1
	PD-L1
	swine diseases
発行日	2021-08-20
出版者	John Wiley & Sons
収録物名	Immunity, inflammation and disease
巻(号)	9(4)
ページ	1573-1583
抄録	Introduction: Chronic infections lead to the functional exhaustion of T cells. Exhausted T cells are phenotypically differentiated by the surface expression of the immunoinhibitory receptor, such as programmed death-1 (PD-1). The inhibitory signal is produced by the interaction between PD-i and its PD-ligand 1 (PD-L1) and impairs the effector functions of T cells. However, the expression dynamics of PD-L1 and the immunological functions of the PD-1/ PD-Ll pathway in chronic diseases of pigs are still poorly understood. In this study, we first analyzed the expression of PD-L1 in various chronic infections in pigs, and then evaluated the immune activation by the blocking assay targeting the swine PD-1/PD-L1 pathway. Methods: In the initial experiments, anti-bovine PD-L1 monoclonal anti-bodies (mAbs) were tested for cross-reactivity with swine PD-L1. Subsequently, immunohistochemical analysis was conducted using the anti-PD-L1 mAb. Finally, we assessed the immune activation of swine peripheral blood mononuclear cells (PBMCs) by the blockade with anti-PD-L1 mAb. Results: Several anti-PD-L1 mAbs tested recognized swine PD-Ll-expressing cells. The binding of swine PD-Ll protein to swine PD-1 was inhibited by some of these cross-reactive mAbs. In addition, immunohistochemical analysis revealed that PD-L1 was expressed at the site of infection in chronic infections of pigs. The PD-L1 blockade increased the production of interleukin-2 from swine PBMCs. Conclusions: These findings suggest that the PD-1/PD-L1 pathway could be also involved in immunosuppression in chronic infections in pigs. This study provides a new perspective on therapeutic strategies for chronic diseases in pigs by targeting immunosuppressive pathways.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
EISSN	2050-4527
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1002/iid3.510