Urinary free metanephrines measurement in dogs with adrenal gland diseases using a new simple liquid chromatography tandem mass spectrometry method

Sasaki, Noboru; Ikenaka, Yoshinori; Inoue, Yumiko; Ichise, Takahiro; Nagata, Noriyuki; Ishizuka, Mayumi; Nakayama, Syouta Mm; Nakamura, Kensuke; Takiguchi, Mitsuyoshi

Urinary free metanephrines measurement in dogs with adrenal gland diseases using a new simple liquid chromatography tandem mass spectrometry method

Sasaki, Noboru; Ikenaka, Yoshinori; Inoue, Yumiko et al.

Journal of veterinary medical science, 2021, 83(4), 648-655

Permalink : https://hdl.handle.net/2115/82669

このアイテムのアクセス数:560件（2026-10-15 17:52 集計）

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https://doi.org/10.1292/jvms.20-0508			23

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アクセス権	open access
URI	https://hdl.handle.net/2115/82669
タイトル	Urinary free metanephrines measurement in dogs with adrenal gland diseases using a new simple liquid chromatography tandem mass spectrometry method
著者	Sasaki, Noboru ; Ikenaka, Yoshinori ; Inoue, Yumiko ; Ichise, Takahiro ; Nagata, Noriyuki ; Ishizuka, Mayumi ; Nakayama, Syouta Mm ; Nakamura, Kensuke ; Takiguchi, Mitsuyoshi
e-Rad_Researcher 00754532 著者名 Sasaki, Noboru
e-Rad_Researcher 40543509 著者名 Ikenaka, Yoshinori
著者名 Inoue, Yumiko
著者名 Ichise, Takahiro
著者名 Nagata, Noriyuki
e-Rad_Researcher 50332474 著者名 Ishizuka, Mayumi
著者名 Nakayama, Syouta Mm
e-Rad_Researcher 80625898 著者名 Nakamura, Kensuke
e-Rad_Researcher 70261336 著者名 Takiguchi, Mitsuyoshi
言語	英語
キーワード	canine
	liquid chromatography tandem mass spectrometry
	normetanephrine
	pheochromocytoma
	urinary metanephrines
発行日	2021-05-05
出版者	公益社団法人日本獣医学会 (The Japanese Society of Veterinary Science)
収録物名	Journal of veterinary medical science
巻(号)	83(4)
ページ	648-655
抄録	Measurement of urinary metanephrines in spot samples is used for the diagnosis of canine pheochromocytoma (PC). We describe a simple analytical method based on liquid chromatography tandem mass spectrometry ( LC- MS/MS) for measuring free metanephrine (MN) and normetanephrine (NMN) in spot urine samples. Using the developed method, we evaluated the stability of urinary free-MN and free-NMN at various storing conditions. In addition, we assessed the feasibility of urinary free-MN and -NMN measurement for diagnosing PC. Urine samples were mixed with stable isotope internal standards and thereafter purified by ultrafiltration. The purified samples were analyzed by LC-MS/MS in the multiple reaction monitoring mode after separation on a multimode octa decyl silyl column. The coefficient of variation of free-MN and -NMN measurement was 7.6% and 5.5%, respectively. The linearity range was 0.5-10 mu g/l for both analytes. Degradation was less than 10% for both analytes under any of the storage conditions. The median free-NMN ratio to creatinine of 9 PC dogs (595, range 144-47,961) was significantly higher (P< 0.05) than that of 13 dogs with hypercortisolism (125, range 52-224) or 15 healthy dogs (85, range 50-117). The developed method is simple and may not require acidification of spot urine. The results of this preliminary retrospective study suggest that the measurement of urinary free metanephrines is a promising tool for diagnosing canine PC.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
PISSN	0916-7250
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1292/jvms.20-0508