Selection of reference genes for quantitative PCR analysis in poultry red mite (Dermanyssus gallinae)

Ariizumi, Takuma; Murata, Shiro; Fujisawa, Sotaro; Isezaki, Masayoshi; Maekawa, Naoya; Okagawa, Tomohiro; Sato, Takumi; Oishi, Eiji; Taneno, Akira; Konnai, Satoru; Ohashi, Kazuhiko

Selection of reference genes for quantitative PCR analysis in poultry red mite (Dermanyssus gallinae)

Ariizumi, Takuma; Murata, Shiro; Fujisawa, Sotaro et al.

Journal of veterinary medical science, 2021, 83(4), 558-565

Permalink : https://hdl.handle.net/2115/82670

このアイテムのアクセス数:507件（2026-10-11 23:52 集計）

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https://doi.org/10.1292/jvms.20-0677			20

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アクセス権	open access
URI	https://hdl.handle.net/2115/82670
タイトル	Selection of reference genes for quantitative PCR analysis in poultry red mite (Dermanyssus gallinae)
著者	Ariizumi, Takuma ; Murata, Shiro ; Fujisawa, Sotaro ; Isezaki, Masayoshi ; Maekawa, Naoya ; Okagawa, Tomohiro ; Sato, Takumi ; Oishi, Eiji ; Taneno, Akira ; Konnai, Satoru ; Ohashi, Kazuhiko
著者名 Ariizumi, Takuma
e-Rad_Researcher 10579163 著者名 Murata, Shiro
著者名 Fujisawa, Sotaro
著者名 Isezaki, Masayoshi
e-Rad_Researcher 70829035 著者名 Maekawa, Naoya
e-Rad_Researcher 80829036 著者名 Okagawa, Tomohiro
著者名 Sato, Takumi
著者名 Oishi, Eiji
著者名 Taneno, Akira
e-Rad_Researcher 40396304 著者名 Konnai, Satoru
e-Rad_Researcher 90250498 著者名 Ohashi, Kazuhiko
言語	英語
キーワード	apolipophorins
	Dermanyssus gallinae
	Elongation factor 1-alpha
	poultry red mite
	reference genes
発行日	2021-04-09
出版者	公益社団法人日本獣医学会 (The Japanese Society of Veterinary Science)
収録物名	Journal of veterinary medical science
巻(号)	83(4)
ページ	558-565
抄録	Poultry red mites (PRMs, Dermanyssus gallinae) are harmful ectoparasites that affect farmed chickens and cause serious economic losses in the poultry industry worldwide. Acaricides are used for PRM control; however, some PRMs have developed acaricide-resistant properties, which have indicated the need for different approaches for PRM control.Therefore, it is necessary to elucidate the biological status of PRMs to develop alternative PRM control strategies. Quantitative polymerase chain reaction (qPCR) allows analysis of the biological status at the transcript level. However, reference genes are preferable for accurate comparison of expression level changes given the large variation in the quality of the PRM samples collected in each farm. This study aimed to identify candidate reference genes with stable expression levels in the different blood feeding states and life stages of PRMs. First, we selected candidates based on the following criteria: sufficient expression intensity and no significant expression difference between fed and starved states. We selected and characterized seven candidate reference genes. Among them, we evaluated the gene expression stability between the starved and fed states using RefFinder; moreover, we compared their expression levels in each life-stage and identified two reference genes, Elongation factor 1-alpha (ELF1A)-like and apolipophorins-like. Finally, we evaluated the utility of the candidates as reference genes, and the use of ELF1A-like and apolipophorins-like successfully normalized ATP synthase subunit g -like gene expression. Thus, ELF1A-like and apolipophorins-like could be suitable reference genes in PRMs.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
PISSN	0916-7250
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1292/jvms.20-0677