Characteristics of Classical Swine Fever Virus Variants Derived from Live Attenuated GPE(-) Vaccine Seed

Kim, Taksoo; Huynh, Loc Tan; Hirose, Shizuka; Igarashi, Manabu; Hiono, Takahiro; Isoda, Norikazu; Sakoda, Yoshihiro

Characteristics of Classical Swine Fever Virus Variants Derived from Live Attenuated GPE(-) Vaccine Seed

Kim, Taksoo; Huynh, Loc Tan; Hirose, Shizuka et al.

Viruses, 2021, 13(8), 1672

Permalink : https://hdl.handle.net/2115/82879

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論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/82879
タイトル	Characteristics of Classical Swine Fever Virus Variants Derived from Live Attenuated GPE(-) Vaccine Seed
著者	Kim, Taksoo ; Huynh, Loc Tan ; Hirose, Shizuka ; Igarashi, Manabu ; Hiono, Takahiro ; Isoda, Norikazu ; Sakoda, Yoshihiro
著者名 Kim, Taksoo
著者名 Huynh, Loc Tan
著者名 Hirose, Shizuka
e-Rad_Researcher 10374240 著者名 Igarashi, Manabu
e-Rad_Researcher 00775819 著者名 Hiono, Takahiro
e-Rad_Researcher 80615732 著者名 Isoda, Norikazu
e-Rad_Researcher 40333637 著者名 Sakoda, Yoshihiro
言語	英語
キーワード	classical swine fever virus
	GPE(-)
	live attenuated vaccine
	vaccine seed
	variant
	NS5B
	type I interferon
発行日	2021-08
出版者	MDPI
収録物名	Viruses
巻(号)	13(8)
ページ	1672
抄録	The GPE(-) strain is a live attenuated vaccine for classical swine fever (CSF) developed in Japan. In the context of increasing attention for the differentiating infected from vaccinated animals (DIVA) concept, the achievement of CSF eradication with the GPE(-) proposes it as a preferable backbone for a recombinant CSF marker vaccine. While its infectious cDNA clone, vGPE(-), is well characterized, 10 amino acid substitutions were recognized in the genome, compared to the original GPE(-) vaccine seed. To clarify the GPE(-) seed availability, this study aimed to generate and characterize a clone possessing the identical amino acid sequence to the GPE(-) seed. The attempt resulted in the loss of the infectious GPE(-) seed clone production due to the impaired replication by an amino acid substitution in the viral polymerase NS5B. Accordingly, replication-competent GPE(-) seed variant clones were produced. Although they were mostly restricted to propagate in the tonsils of pigs, similarly to vGPE(-), their type I interferon-inducing capacity was significantly lower than that of vGPE(-). Taken together, vGPE(-) mainly retains ideal properties for the CSF vaccine, compared with the seed variants, and is probably useful in the development of a CSF marker vaccine.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
PISSN	1999-4915
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.3390/v13081672