Bovine hepatocyte growth factor and its receptor c-Met: cDNA cloning and expression analysis in the mammary gland

Yamaji, Daisuke; Kimura, Kazuhiro; Watanabe, Atsushi; Kon, Yasuhiro; Iwanaga, Toshihiko; Soliman, Mohamed M.; Ahmed, Mohamed M.; Saito, Masayuki

Bovine hepatocyte growth factor and its receptor c-Met: cDNA cloning and expression analysis in the mammary gland

Yamaji, Daisuke; Kimura, Kazuhiro; Watanabe, Atsushi et al.

Domestic Animal Endocrinology, 2006, 30(3), 239-246

Permalink : https://hdl.handle.net/2115/8299

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アクセス権	open access
URI	https://hdl.handle.net/2115/8299
タイトル	Bovine hepatocyte growth factor and its receptor c-Met: cDNA cloning and expression analysis in the mammary gland
著者	Yamaji, Daisuke ; Kimura, Kazuhiro ; Watanabe, Atsushi ; Kon, Yasuhiro ; Iwanaga, Toshihiko ; Soliman, Mohamed M. ; Ahmed, Mohamed M. ; Saito, Masayuki
著者名 Yamaji, Daisuke
e-Rad_Researcher 30192561 著者名 Kimura, Kazuhiro
著者名 Watanabe, Atsushi
e-Rad_Researcher 10178402 著者名 Kon, Yasuhiro
e-Rad_Researcher 10160128 著者名 Iwanaga, Toshihiko
著者名 Soliman, Mohamed M.
著者名 Ahmed, Mohamed M.
著者名 Saito, Masayuki
言語	英語
キーワード	HGF
	c-Met
	cDNA cloning
	mammary gland
	epithelial cells
発行日	2006-03
出版者	Elsevier
収録物名	Domestic Animal Endocrinology
巻(号)	30(3)
ページ	239-246
記述	http://www.sciencedirect.com/science/journal/07397240
抄録	Hepatocyte growth factor/scatter factor (HGF/SF) is a pleiotropic cytokine that plays a crucial role in the embryonic and postnatal development of various organs including the mammary gland. We cloned bovine HGF and its c-Met receptor cDNAs, and examined their expression during mammary gland development in dairy cows. The 2.5-kbp HGF cDNA clone contained a 2,190 bp open reading frame coding a 730 amino acid protein, while the 4.8-kbp c-Met cDNA clone contained a 4,152 bp open reading frame coding a 1,384 amino acid protein. The bovine HGF and c-Met sequences exhibited more than 87% identity with those of other mammals. RT-PCR analysis revealed ubiquitous expression of both HGF and c-Met mRNAs in various bovine tissues tested. HGF mRNA was detected only in the inactive stage of bovine mammary gland development and not in the developing, lactating, and involuting stages, while c-Met mRNA was detected in the inactive and involuting stages. Immunohistochemical analysis demonstrated that the c-Met protein was found on mammary epithelial cells in the inactive, developing, and involuting stages, and on myoepithelial cells in all stages. These results suggest pivotal roles of HGF and c-Met in the development of bovine mammary gland.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	0739-7240
関連情報	PMID 16207523
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.domaniend.2005.08.005