Analysis of serum lysophosphatidylethanolamine levels in patients with non-alcoholic fatty liver disease by liquid chromatography-tandem mass spectrometry

Yamamoto, Yusuke; Sakurai, Toshihiro; Chen, Zhen; Furukawa, Takayuki; Gowda, Siddabasave Gowda B.; Wu, Yue; Nouso, Kazuhiro; Fujii, Yuki; Yoshikawa, Yuki; Chiba, Hitoshi; Hui, Shu-Ping

Analysis of serum lysophosphatidylethanolamine levels in patients with non-alcoholic fatty liver disease by liquid chromatography-tandem mass spectrometry

Yamamoto, Yusuke; Sakurai, Toshihiro; Chen, Zhen et al.

Analytical and bioanalytical chemistry, 2020, 413(1), 245-254

Permalink : https://hdl.handle.net/2115/83041

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アクセス権	open access
URI	https://hdl.handle.net/2115/83041
タイトル	Analysis of serum lysophosphatidylethanolamine levels in patients with non-alcoholic fatty liver disease by liquid chromatography-tandem mass spectrometry
著者	Yamamoto, Yusuke ; Sakurai, Toshihiro ; Chen, Zhen ; Furukawa, Takayuki ; Gowda, Siddabasave Gowda B. ; Wu, Yue ; Nouso, Kazuhiro ; Fujii, Yuki ; Yoshikawa, Yuki ; Chiba, Hitoshi ; Hui, Shu-Ping
著者名 Yamamoto, Yusuke
e-Rad_Researcher 60707602 著者名 Sakurai, Toshihiro
e-Rad_Researcher 60802634 著者名 Chen, Zhen
e-Rad_Researcher 30754642 著者名 Furukawa, Takayuki
著者名 Gowda, Siddabasave Gowda B.
著者名 Wu, Yue
e-Rad_Researcher 10314668 著者名 Nouso, Kazuhiro
著者名 Fujii, Yuki
著者名 Yoshikawa, Yuki
e-Rad_Researcher 70197622 著者名 Chiba, Hitoshi
e-Rad_Researcher 90337030 著者名 Hui, Shu-Ping
言語	英語
キーワード	Lysophosphatidylethanolamine
	LysoPE
	Liquid chromatography-tandem mass spectrometry
	Chemical synthesis
	Non-alcoholic fatty liver disease
発行日	2020-10-22
出版者	Springer
収録物名	Analytical and bioanalytical chemistry
巻(号)	413(1)
ページ	245-254
抄録	Lysophosphatidylethanolamines (LysoPEs) are the partial hydrolysis products of phosphatidylethanolamine. Despite the unique in vitro bioactivities of LysoPEs, there are limited reports on the pathophysiological role of LysoPEs in the serum, due to the lack of sensitive analytical methods for determination of each molecular species in clinical samples. Herein, we developed a highly sensitive quantitative method to profile the serum LysoPE species by liquid chromatography-tandem mass spectrometry (LC-MS/MS) with selected reaction monitoring (SRM). The internal standard (IS), chemically synthesized in-house, and the lineup of seven major LysoPE species were used in this study. The limits of detection and quantification for each LysoPE species ranged within 0.5-3.3 pmol/mL and 1.0-5.0 pmol/mL, respectively. The combined concentrations of LysoPEs in the serum from healthy subjects (n = 8) and the patients with non-alcoholic fatty liver diseases (NAFLD) including simple steatosis (SS, n = 9) and non-alcoholic steatohepatitis (NASH, n = 27) were 18.030 +/- 3.832, 4.867 +/- 1.852, and 5.497 +/- 2.495 nmol/mL, respectively. The combined and individual concentrations of LysoPEs, except for LysoPE 18:0, significantly decreased in the patients with NAFLD compared with those for the healthy subjects. However, no significant difference was observed between the SS and NASH groups. Our proposed LC-MS/MS method is valid and has advantages of small sample volume, high sensitivity, and simultaneous absolute quantitation for multiple molecular species. This method may enable diagnostic evaluation and elucidation of the as-yet uncovered pathophysiological role of LysoPEs.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	This is a post-peer-review, pre-copyedit version of an article published in Analytical and bioanalytical chemistry. The final authenticated version is available online at: http://dx.doi.org/10.1007/s00216-020-02996-9.
PISSN	1618-2642
関連情報	PMID 33090255
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1007/s00216-020-02996-9