Interaction of the plasmid-encoded quinolone resistance protein QnrB19 with Salmonella Typhimurium DNA gyrase

Pachanon, Ruttana; Koide, Kentaro; Kongsoi, Siriporn; Nakajima, Chie; Kapalamula, Thoko Flav; Suthienkul, Orasa; Suzuki, Yasuhiko

Interaction of the plasmid-encoded quinolone resistance protein QnrB19 with Salmonella Typhimurium DNA gyrase

Pachanon, Ruttana; Koide, Kentaro; Kongsoi, Siriporn et al.

Journal of infection and chemotherapy, 2020, 26(11), 1139-1145

Permalink : https://hdl.handle.net/2115/83155

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論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/83155
タイトル	Interaction of the plasmid-encoded quinolone resistance protein QnrB19 with Salmonella Typhimurium DNA gyrase
著者	Pachanon, Ruttana ; Koide, Kentaro ; Kongsoi, Siriporn ; Nakajima, Chie ; Kapalamula, Thoko Flav ; Suthienkul, Orasa ; Suzuki, Yasuhiko
著者名 Pachanon, Ruttana
著者名 Koide, Kentaro
著者名 Kongsoi, Siriporn
e-Rad_Researcher 60435964 著者名 Nakajima, Chie
著者名 Kapalamula, Thoko Flav
著者名 Suthienkul, Orasa
e-Rad_Researcher 90206540 著者名 Suzuki, Yasuhiko
言語	英語
キーワード	PMQR
	Quinolone resistance
	QnrB19
	Salmonella typhimurium
	DNA gyrase
発行日	2020-11-01
出版者	Elsevier
収録物名	Journal of infection and chemotherapy
巻(号)	26(11)
ページ	1139-1145
抄録	Background: Plasmid-encoded quinolone resistance protein Qnr is an important factor in bacterial resistance to quinolones. Qnr interacts with DNA gyrase and reduces susceptibility to quinolones. The gene qnr likely spreads rapidly among Enterobacteriaceae via horizontal gene transfer. Though the vast amounts of epidemiological data are available, molecular details of the contribution of QnrB19, the predominant Qnr in Salmonella spp., to the acquisition of quinolone resistance has not yet been understood well. Objective: We aimed to examine the role of QnrB19 in quinolone resistance acquisition using recombinant Salmonella Typhimurium DNA gyrases and QnrB19. Materials and methods: Recombinant QnrB19 was expressed in E. coli and purified by Ni-NTA agarose column chromatography. DNA supercoiling activities of recombinant Salmonella Typhimurium DNA gyrase were assessed with or without QnrB19 under the existence of three quinolones to measure IC(50)s, the concentration of each quinolone required for 50% inhibition in vitro. Results: The IC(50)s of norfloxacin, ciprofloxacin and nalidixic acid against DNA gyrases were measured to be 0.30, 0.16 and 17.7 mu g/mL, respectively. The addition of QnrB19 increased the IC(50)s of norfloxacin and ciprofloxacin to be 0.81 and 0.48 mu g/mL, respectively, where no effect of QnrB19 was observed on the IC50 of nalidixic acid. Conclusion: QnrB19 was shown for the first time in vitro to have ability to grant non-classical quinolone resistance to S. Typhimurium DNA gyrase. Structural insight on quinolones in this study may contribute to investigate drugs useful for preventing the spread of plasmid carrying PMQR along with other factors associating with antimicrobial resistance in S. Typhimurium and other bacteria. (C) 2020 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases. Published by Elsevier Ltd. All rights reserved.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	© 2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	1341-321X
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.jiac.2020.06.002