Utilizing attached hard ticks as pointers to the risk of infection by Babesia and Theileria species in sika deer (Cervus nippon yesoensis), in Japan

Elzahara, Elbaz; Mohamed Abdallah Mohamed, Moustafa; Kyunglee, Lee; Ching, Alice Lau Ching; Shimozuru, Michito; Sashika, Mariko; Nakao, Ryo; El-khodery, Sabry Ahmed; Tsubota, Toshio

Utilizing attached hard ticks as pointers to the risk of infection by Babesia and Theileria species in sika deer (Cervus nippon yesoensis), in Japan

Elzahara, Elbaz; Mohamed Abdallah Mohamed, Moustafa; Kyunglee, Lee et al.

Experimental and Applied Acarology, 2020, 82(3), 411-429

Permalink : https://hdl.handle.net/2115/83179

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アクセス権	open access
URI	https://hdl.handle.net/2115/83179
タイトル	Utilizing attached hard ticks as pointers to the risk of infection by Babesia and Theileria species in sika deer (Cervus nippon yesoensis), in Japan
著者	Elzahara, Elbaz ; Mohamed Abdallah Mohamed, Moustafa ; Kyunglee, Lee ; Ching, Alice Lau Ching ; Shimozuru, Michito ; Sashika, Mariko ; Nakao, Ryo ; El-khodery, Sabry Ahmed ; Tsubota, Toshio
著者名 Elzahara, Elbaz
著者名 Mohamed Abdallah Mohamed, Moustafa
著者名 Kyunglee, Lee
著者名 Ching, Alice Lau Ching
著者名 Shimozuru, Michito
著者名 Sashika, Mariko
著者名 Nakao, Ryo
著者名 El-khodery, Sabry Ahmed
e-Rad_Researcher 10207441 著者名 Tsubota, Toshio
言語	英語
キーワード	Babesia
	Hard ticks
	Reverse line blot
	Sika deer
	Theileria
発行日	2020-11
出版者	Springer
収録物名	Experimental and Applied Acarology
巻(号)	82(3)
ページ	411-429
抄録	Ticks are hematophagous ectoparasites that have a significant impact on their animal hosts. Along with mosquitoes, they are the main arthropod vectors of disease agents in domestic animals, wildlife and humans. To investigate the occurrence and prevalence of piroplasmids in ticks, DNA was extracted from 519 hard ticks collected from 116 hunted Hokkaido sika deer (Cervus nippon yesoensis). The success of the DNA extraction was confirmed by touchdown PCR targeting the mitochondrial 16S rDNA gene of ticks. Touchdown PCR and reverse line blot (RLB) hybridization targeting the 18S rRNA gene were used to detect 14 piroplasm species. All hard ticks parasitizing Hokkaido sika deer were identified as belonging to the generaIxodesandHaemaphysalis. In total 163 samples (31.4%) were positive forBabesiaandTheileriaspp. among tick species according to RLB hybridization. Tick DNA hybridized to the oligonucleotide probes ofTheileriasp.Thrivae (27.0% of ticks; 140/519),Theileria capreoli(10.6%; 55/519),Babesia divergens-like (1.7%; 9/519),Babesiasp. (Bab-SD) (0.6%; 3/519),Babesia microtiU.S. (0.4%; 2/519), andB. microtiHobetsu (0.4%; 2/519). The partial sequencing and phylogenetic analyses of the 18S rRNA gene confirmed the RLB hybridization results. Further investigations are needed to reveal the epidemiology and respective vectors of these pathogens.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	This is a post-peer-review, pre-copyedit version of an article published in Experimental and Applied Acarology. The final authenticated version is available online at: https://doi.org/10.1007/s10493-020-00551-w
PISSN	0168-8162
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1007/s10493-020-00551-w