Molecular detection of apicomplexan protozoa in Hokkaido brown bears (Ursus arctos yesoensis) and Japanese black bears (Ursus thibetanus japonicus)

Mohamed Abdallah Mohamed, Moustafa; Ayaka, Sasaki; Michito, Shimozuru; Nakao, Ryo; Sashika, Mariko; Yamazaki, Koji; Koike, Shinsuke; Tanaka, Junpei; Tamatani, Hiroo; Yamanaka, Masami; Ishinazaka, Tsuyoshi; Tsubota, Toshio

Molecular detection of apicomplexan protozoa in Hokkaido brown bears (Ursus arctos yesoensis) and Japanese black bears (Ursus thibetanus japonicus)

Mohamed Abdallah Mohamed, Moustafa; Ayaka, Sasaki; Michito, Shimozuru et al.

Parasitology Research, 2020, 119(11), 3739-3753

Permalink : https://hdl.handle.net/2115/83181

このアイテムのアクセス数:583件（2026-08-14 16:39 集計）

閲覧可能ファイル

ファイル	フォーマット	サイズ	閲覧回数	説明
Clean file_Para.res.manuscript_co-authors addition200904	pdf	440 KB	484

論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

アクセス権	open access
URI	https://hdl.handle.net/2115/83181
タイトル	Molecular detection of apicomplexan protozoa in Hokkaido brown bears (Ursus arctos yesoensis) and Japanese black bears (Ursus thibetanus japonicus)
著者	Mohamed Abdallah Mohamed, Moustafa ; Ayaka, Sasaki ; Michito, Shimozuru ; Nakao, Ryo ; Sashika, Mariko ; Yamazaki, Koji ; Koike, Shinsuke ; Tanaka, Junpei ; Tamatani, Hiroo ; Yamanaka, Masami ; Ishinazaka, Tsuyoshi ; Tsubota, Toshio
著者名 Mohamed Abdallah Mohamed, Moustafa
著者名 Ayaka, Sasaki
著者名 Michito, Shimozuru
著者名 Nakao, Ryo
著者名 Sashika, Mariko
著者名 Yamazaki, Koji
著者名 Koike, Shinsuke
著者名 Tanaka, Junpei
著者名 Tamatani, Hiroo
著者名 Yamanaka, Masami
著者名 Ishinazaka, Tsuyoshi
e-Rad_Researcher 10207441 著者名 Tsubota, Toshio
言語	英語
キーワード	Bears
	RLB
	Apicomplexan protozoa
	Babesia microti
	Cytauxzoon
発行日	2020-11-01
出版者	Springer
収録物名	Parasitology Research
巻(号)	119(11)
ページ	3739-3753
抄録	Many tick-borne pathogens (TBPs) are present in wildlife. The objective of this study is to reveal the role of wild bears in maintaining TBPs. A total of 49 brown bears (Ursus arctos yesoensis) from Hokkaido, and 18 Japanese black bears (Ursus thibetanus japonicus) from Tochigi, and 66 Japanese black bears from Nagano were examined by two molecular methods, reverse line blot (RLB) hybridization, and nested PCR. A total of 5 TBPs (Hepatozoon ursi,Babesiasp. UR2-like group,Cytauxzoonsp. UR1,Babesiasp. UR1, andBabesia microti) were detected from bear blood DNA samples.B. microtiwas detected from blood DNA samples of Japanese black bear for the first time, with the prevalence of 6.0% (5/84). Out of detected pathogens,H. ursi,Babesiasp. UR2-like pathogens, andCytauxzoonsp. UR1 were considered as three of the most prevalent TBPs in bears. The prevalence ofH. ursiwere significantly higher in Japanese black bear (0% vs 96.4%) while that ofBabesiasp. UR2-like group was higher in Hokkaido brown bears (89.8% vs 40.5%). The prevalence ofBabesiasp. UR1 were significantly higher in Japanese black bears from Tochigi (44.4%), comparing with those from Nagano (18.2%). The prevalence of the detected TBPs were significantly higher in adult bears, comparing with those in younger bears. The present study suggests that Japanese bear species contribute in the transmission of several TBPs in Japan. The expanding distribution of bears might cause the accidental transmission of TBPs to humans and domestic animals.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	This is a post-peer-review, pre-copyedit version of an article published in Parasitology Research. The final authenticated version is available online at: https://doi.org/10.1007/s00436-020-06873-3
PISSN	0932-0113
EISSN	1432-1955
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1007/s00436-020-06873-3