Isolation and Characterization of Antimicrobial-Resistant Escherichia coli from Retail Meats from Roadside Butcheries in Uganda

Okubo, Torahiko; Yossapol, Montira; Ikushima, Shiori; Kakooza, Steven; Wampande, Eddie M.; Asai, Tetsuo; Tsuchida, Sayaka; Ohya, Kenji; Maruyama, Fumito; Kabasa, John D.; Ushida, Kazunari

Isolation and Characterization of Antimicrobial-Resistant Escherichia coli from Retail Meats from Roadside Butcheries in Uganda

Okubo, Torahiko; Yossapol, Montira; Ikushima, Shiori et al.

Foodborne Pathogens and Disease, 2020, 17(11), 666-671

Permalink : https://hdl.handle.net/2115/83194

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アクセス権	open access
URI	https://hdl.handle.net/2115/83194
タイトル	Isolation and Characterization of Antimicrobial-Resistant Escherichia coli from Retail Meats from Roadside Butcheries in Uganda
著者	Okubo, Torahiko ; Yossapol, Montira ; Ikushima, Shiori ; Kakooza, Steven ; Wampande, Eddie M. ; Asai, Tetsuo ; Tsuchida, Sayaka ; Ohya, Kenji ; Maruyama, Fumito ; Kabasa, John D. ; Ushida, Kazunari
e-Rad_Researcher 90762196 著者名 Okubo, Torahiko
著者名 Yossapol, Montira
著者名 Ikushima, Shiori
著者名 Kakooza, Steven
著者名 Wampande, Eddie M.
e-Rad_Researcher 10509764 著者名 Asai, Tetsuo
e-Rad_Researcher 40734687 著者名 Tsuchida, Sayaka
e-Rad_Researcher 50402219 著者名 Ohya, Kenji
e-Rad_Researcher 30423122 著者名 Maruyama, Fumito
著者名 Kabasa, John D.
e-Rad_Researcher 50183017 著者名 Ushida, Kazunari
言語	英語
キーワード	retail meat
	roadside butchery
	Uganda
	E. coli
発行日	2020-11-06
出版者	Mary Ann Liebert
収録物名	Foodborne Pathogens and Disease
巻(号)	17(11)
ページ	666-671
抄録	Retail meats are one of the main routes for spreading antimicrobial-resistant bacteria (ARB) from livestock to humans through the food chain. In African countries, retail meats are often sold at roadside butcheries without chilling or refrigeration. Retail meats in those butcheries are suspected to be contaminated by ARB, but it was not clear. In this study, we tested for the presence of antimicrobial-resistantEscherichia colifrom retail meats (n = 64) from roadside butcheries in Kampala, Uganda. The meat surfaces were swabbed and inoculated on PetriFilm SEC agar to isolateE. coli. We successfully isolatedE. colifrom 90.6% of these retail meat samples. We identified the phylogenetic type, antimicrobial susceptibility, and antimicrobial resistance genes prevalence between retail meat isolates (n = 89). Phylogenetic type B1 was identified from 70.8% of the retail meat isolates, suggesting that the isolates originated primarily from fecal contamination during meat processing. Tetracycline (TET)-resistant isolates withtetAand/ortetBgene(s) were the most frequently detected (28.1%), followed by ampicillin (AMP) resistance genes withbla(TEM)(15.7%,) and sulfamethoxazole-trimethoprim (SXT) resistance genes withsul2(15.7%). No extended-spectrum beta-lactamase-producing isolates were detected. A conjugation assay showed that resistance to AMP, TET, and SXT could be simultaneously transferred to recipients. These findings suggest that antimicrobial-resistantE. colican easily be transferred from farms to tables from retail meats obtained from roadside butcheries.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Final publication is available from Mary Ann Liebert, Inc., publishers https://doi.org/10.1089/fpd.2020.2796
PISSN	1535-3141
EISSN	1556-7125
関連情報	PMID 32551973
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1089/fpd.2020.2796