Histone chaperone-mediated co-expression assembly of tetrasomes and nucleosomes

Okimune, Kei-ichi; Hataya, Shogo; Matsumoto, Kazuki et al.

Febs open bio, 2021, 11(11), 2912-2920

Permalink : https://hdl.handle.net/2115/83316

このアイテムのアクセス数:496件（2026-08-26 02:54 集計）

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https://doi.org/10.1002/2211-5463.13311     38

論文情報

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アクセス権
open access
URI
https://hdl.handle.net/2115/83316
タイトル
Histone chaperone-mediated co-expression assembly of tetrasomes and nucleosomes
著者
Okimune, Kei-ichi ; Hataya, Shogo ; Matsumoto, Kazuki ; Ushirogata, Kanako ; Banko, Petra ; Takeda, Seiji ; Takasuka, Taichi E.
著者名
Okimune, Kei-ichi
著者名
Hataya, Shogo
著者名
Matsumoto, Kazuki
著者名
Ushirogata, Kanako
著者名
Banko, Petra
著者名
Takeda, Seiji
e-Rad_Researcher
70748409
著者名
Takasuka, Taichi E.
言語
英語
キーワード
nucleosome
tetrasome
NAP1
wheat germ cell-free synthesis
AFM
発行日
2021-11
出版者
John Wiley & Sons
収録物名
Febs open bio
巻(号)
11(11)
ページ
2912-2920
抄録
The nucleosome, a basic unit of chromatin found in all eukaryotes, is thought to be assembled through the orchestrated activity of several histone chaperones and chromatin assembly factors in a stepwise manner, proceeding from tetrasome assembly, to H2A/H2B deposition, and finally to formation of the mature nucleosome. In this study, we demonstrate chaperone-mediated assembly of both tetrasomes and nucleosomes on the well-defined Widom 601 positioning sequence using a co-expression/reconstitution wheat germ cell-free system. The purified tetrasomes and nucleosomes were positioned around the center of a given sequence. The heights and diameters were measured by atomic force microscopy. Together with the reported unmodified native histones produced by the wheat germ cell-free platform, our method is expected to be useful for downstream applications in the field of chromatin research.
資源タイプ
学術雑誌論文
出版タイプ
Version of Record
PISSN
2211-5463
関連情報 (isIdenticalTo)
出版社版DOI
https://doi.org/10.1002/2211-5463.13311