Amelioration of butylated hydroxytoluene against inorganic mercury induced cytotoxicity and mitochondrial apoptosis in PC12 cells via antioxidant effects

Hossain, Kaniz Fatima Binte; Hosokawa, Toshiyuki; Saito, Takeshi; Kurasaki, Masaaki

Amelioration of butylated hydroxytoluene against inorganic mercury induced cytotoxicity and mitochondrial apoptosis in PC12 cells via antioxidant effects

Hossain, Kaniz Fatima Binte; Hosokawa, Toshiyuki; Saito, Takeshi et al.

Food and Chemical Toxicology, 2020, 146, 111819

Permalink : https://hdl.handle.net/2115/83396

このアイテムのアクセス数:705件（2026-08-26 02:50 集計）

閲覧可能ファイル

ファイル	フォーマット	サイズ	閲覧回数	説明
Food Chem. Toxicol. v146_R1	pdf	0.98 MB	655

論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

アクセス権	open access
URI	https://hdl.handle.net/2115/83396
タイトル	Amelioration of butylated hydroxytoluene against inorganic mercury induced cytotoxicity and mitochondrial apoptosis in PC12 cells via antioxidant effects
著者	Hossain, Kaniz Fatima Binte ; Hosokawa, Toshiyuki ; Saito, Takeshi ; Kurasaki, Masaaki
著者名 Hossain, Kaniz Fatima Binte
e-Rad_Researcher 00157025 著者名 Hosokawa, Toshiyuki
e-Rad_Researcher 40153811 著者名 Saito, Takeshi
e-Rad_Researcher 80161727 著者名 Kurasaki, Masaaki
言語	英語
キーワード	Oxidative stress
	Intrinsic apoptosis
	Nrf2
	Heme oxygenege 1
	ERK1
	DNA fragmentation
発行日	2020-12
出版者	Elsevier
収録物名	Food and Chemical Toxicology
巻(号)	146
ページ	111819
抄録	Mercury (Hg) is a toxic metal, well-known for its dangerous health effects on human. Butylated hydroxytoluene (BHT) is a phenolic component generally consumed as a food additive as an antioxidant. However, BHT induced antioxidant properties against heavy metals-influenced toxicity are little studied. We hypothesized that BHT has a regulatory effect on Hg-induced cytotoxicity. The objective of this research was to assess the protecting effects of BHT against inorganic Hg (iHg)-toxicity in PC12 cells, where cells were treated with/without HgCl2 (Hg2+) (5 mu M) and BHT (100 mu M) for 48 h and analyzed further. Cells treated by Hg caused a significant cell viability reduction, membrane damage, glutathione reduction, DNA fragmentation, ROS generation, with suppressed expressions of akt, mTOR, ERK1, Nrf2 and HO1; and elevated apoptotic expressions of p53, Bax, cytochrome c and active caspase 3. However, BHT and Hg2+ co-exposure showed prevention against Hg2+-toxicity by improving GSH content and inhibiting ROS generation and oxidative stress mediated damages. Additionally, BHT co-treatment inverted the pro-apoptotic proteins by augmenting pro-survival regulatory proteins akt, mTOR, ERK1, Nrf2 and HO1. These findings proved that BHT inhibits Hg2+-toxicity, hindering ROS generation and intrinsic apoptosis, via enhancing glutathione and antioxidants; and suggested BHT implications as therapeutic.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	© 2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	0278-6915
NCID	AA10627174
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.fct.2020.111819