open access

https://hdl.handle.net/2115/83415

Effects of methyl methacrylate on the excitability of the area postrema neurons in rats

英語

Vagotomy

2020-12

Elsevier

Journal of oral biosciences

62(4)

306-309

Objectives: The aim of the present study is to demonstrate the effects of inhaled methyl methacrylate (MMA) on the excitability of neurons in the area postrema (AP). We also investigated the relation between vagal afferent inputs and responding cells in the AP. Methods: We set up two groups of experimental animals, such as rats inhaling MMA and rats inhaling room air. To visualize the changes of AP neuron excitability after inhalation of MMA for 90 min, c-Fos protein expression was identified and quantified by immunohistochemical analysis. Some rats receiving ventral gastric branch vagotomy were also subjected to the abovementioned experiment. Results: The number of c-Fos-immunoreactive (Fos-ir) cells in the MMA group was more than six times greater than that of the control group (statistically significant, p < 0.01). In vagotomized rats inhaling MMA, markedly smaller number of Fos-ir cells was identified in the AP compared to that of rats inhaling MMA without vagotomy. Conclusions: These results indicate that inhalation of MMA increases neuronal excitability in the AP, suggesting that vagal afferent inputs are involved in the induction mechanism of Fos-ir cells by MMA. (C) 2020 Japanese Association for Oral Biology. Published by Elsevier B.V. All rights reserved.

学術雑誌論文

Accepted Manuscript

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© 2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license

1349-0079