Characterization of Mutations Associated with Streptomycin Resistance in Multidrug-Resistant Mycobacterium tuberculosis in Zambia

Bwalya, Precious; Yamaguchi, Tomoyuki; Solo, Eddie Samuneti; Chizimu, Joseph Yamweka; Mbulo, Grace; Nakajima, Chie; Suzuki, Yasuhiko

Characterization of Mutations Associated with Streptomycin Resistance in Multidrug-Resistant Mycobacterium tuberculosis in Zambia

Bwalya, Precious; Yamaguchi, Tomoyuki; Solo, Eddie Samuneti et al.

Antibiotics-Basel, 2021, 10(10), 1169

Permalink : https://hdl.handle.net/2115/83492

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https://doi.org/10.3390/antibiotics10101169			45

論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/83492
タイトル	Characterization of Mutations Associated with Streptomycin Resistance in Multidrug-Resistant Mycobacterium tuberculosis in Zambia
著者	Bwalya, Precious ; Yamaguchi, Tomoyuki ; Solo, Eddie Samuneti ; Chizimu, Joseph Yamweka ; Mbulo, Grace ; Nakajima, Chie ; Suzuki, Yasuhiko
著者名 Bwalya, Precious
著者名 Yamaguchi, Tomoyuki
著者名 Solo, Eddie Samuneti
著者名 Chizimu, Joseph Yamweka
著者名 Mbulo, Grace
e-Rad_Researcher 60435964 著者名 Nakajima, Chie
e-Rad_Researcher 90206540 著者名 Suzuki, Yasuhiko
言語	英語
キーワード	Mycobacterium tuberculosis
	streptomycin
	multidrug-resistant tuberculosis (MDR-TB)
	spoligotype
	Zambia
発行日	2021-10
出版者	MDPI
収録物名	Antibiotics-Basel
巻(号)	10(10)
ページ	1169
抄録	Streptomycin (STR) is recommended for the management of multidrug-resistant tuberculosis (MDR-TB). Streptomycin resistance-conferring mutation types and frequency are shown to be influenced by genotypes of circulating strains in a population. This study aimed to characterize the mutations in MDR-TB isolates and examine their relationship with the genotypes in Zambia. A total of 138 MDR-TB isolates stored at the University Teaching Hospital Tuberculosis Reference Laboratory in Zambia were analyzed using spoligotyping and sequencing of STR resistance-associated genes. Streptomycin resistance was observed in 65.9% (91/138) of MDR-TB isolates. Mutations in rpsL, rrs, and gidB accounted for 33%, 12.1%, and 49.5%, respectively. Amino acid substitution K43R in rpsL was strongly associated with the CAS1_Kili genotype (p < 0.0001). The combination of three genes could predict 91.2% of STR resistance. Clustering of isolates based on resistance-conferring mutations and spoligotyping was observed. The clustering of isolates suggests that the increase in STR-resistant MDR-TB in Zambia is largely due to the spread of resistant strains from inadequate treatment. Therefore, rapid detection of STR resistance genetically is recommended before its use in MDR-TB treatment in Zambia.</p>
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
PISSN	2079-6382
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.3390/antibiotics10101169